chr7 : 115,229,189 115,229,997
808 bp 120 TFs 1 linked gene
This 808 bp open chromatin element is linked to LINC01392 and is bound by 120 transcription factors.
Linked Genes
1 gene
Gene Expression Dist. to TSS Distance Link type
LINC01392 1.4 kb Proximal Proximity
Chromatin Accessibility

Genome Browser

Genomic view centred on this element. Tracks include ATAC-seq accessibility, RNA-seq expression, TF binding peaks, and element-to-gene loop arcs across the ESC → DE time course.

View limited to ±500 kb around this element.
For the full data, please see the UCSC track linked below.
chr7:115,224,189 – 115,234,997
This element Other elements Gene links ATAC-seq RNA-seq

Transcription factors with ChIP-seq peak overlap or TOBIAS motif footprint evidence at this element.

TF Binding
120 transcription factors
Source
Cell type
AR 3 datasets
ChIP LAPC-4_R1881 GSE148358.AR.LAPC-4_R1881 252 bp overlap
ChIP prostate-cancer_PDX_136 GSE130408.AR.prostate-cancer_PDX_136 250 bp overlap
ChIP prostate_DHT GSE61838.AR.prostate_DHT 189 bp overlap
ATF2 2 datasets
ChIP GM12878 ENCFF521LQJ 327 bp overlap
ChIP GM12878 ENCSR000BQK.ATF2.GM12878 111 bp overlap
BCL6 1 dataset
ChIP SU-DHL-4 GSE119038.BCL6.SU-DHL-4 782 bp overlap
BRD4 7 datasets
ChIP CD4_JG1 GSE33281.BRD4.CD4_JG1 486 bp overlap
ChIP HEK293T GSE39579.BRD4.HEK293T 165 bp overlap
ChIP HeLa GSE151038.BRD4.HeLa 808 bp overlap
ChIP HeLa_JQ1 GSE151038.BRD4.HeLa_JQ1 808 bp overlap
ChIP KOPT-K1 GSE54379.BRD4.KOPT-K1 273 bp overlap
ChIP KOPT-K1_E GSE54379.BRD4.KOPT-K1_E 443 bp overlap
ChIP MM1-S_JQ1_50NM GSE42355.BRD4.MM1-S_JQ1_50NM 120 bp overlap
Bcl11B 1 dataset
Motif DE_12h DE_12h-Bcl11B_MA1989.2 9 bp overlap
CBFB 2 datasets
ChIP GM12878 ENCSR860UHK.CBFB.GM12878 220 bp overlap
ChIP Hep-G2 ENCSR479RZV.CBFB.Hep-G2 142 bp overlap
CDK9 1 dataset
ChIP MM1-S_JQ1_5000NM GSE49224.CDK9.MM1-S_JQ1_5000NM 276 bp overlap
CDX2 3 datasets
ChIP LS180 GSE31939.CDX2.LS180 205 bp overlap
ChIP LS180 GSE31939.CDX2.LS180 146 bp overlap
ChIP LS180_125 GSE31939.CDX2.LS180_125 588 bp overlap
CDX4 1 dataset
Motif DE_12h DE_12h-CDX4_MA1473.2 9 bp overlap
CEBPA 2 datasets
Motif DE_12h DE_12h-CEBPA_MA0102.5 10 bp overlap
ChIP Hep-G2 ERP000209.CEBPA.Hep-G2 155 bp overlap
CEBPB 1 dataset
ChIP HCT-116 ENCSR000BSD.CEBPB.HCT-116 151 bp overlap
CEBPD 1 dataset
Motif DE_12h DE_12h-CEBPD_MA0836.3 8 bp overlap
CEBPG 2 datasets
Motif DE_12h DE_12h-CEBPG_MA0838.1 10 bp overlap
Motif DE_12h DE_12h-CEBPG_MA1636.2 10 bp overlap
CREB1 1 dataset
ChIP GM12878 ENCSR000BUF.CREB1.GM12878 151 bp overlap
CRY2 1 dataset
ChIP U2OS GSE130602.CRY2.U2OS 204 bp overlap
CTCF 1 dataset
ChIP GP5D GSE51234.CTCF.GP5D 436 bp overlap
CTNNB1 2 datasets
ChIP LS180 GSE31939.CTNNB1.LS180 115 bp overlap
ChIP LS180_125 GSE31939.CTNNB1.LS180_125 116 bp overlap
Cebpa 4 datasets
ChIP BLaER1 ENCFF093OYK 432 bp overlap
ChIP BLaER1 ENCFF274GAT 305 bp overlap
ChIP BLaER1 ENCFF346MCV 360 bp overlap
ChIP BLaER1 ENCFF364PUR 371 bp overlap
DBP 1 dataset
Motif DE_12h DE_12h-DBP_MA0639.2 10 bp overlap
E2F3 1 dataset
Motif DE_12h DE_12h-E2F3_MA0469.4 14 bp overlap
FLI1 1 dataset
ChIP SEM GSE117864.FLI1.SEM 139 bp overlap
FOXA1 7 datasets
ChIP CFPAC-1 GSE119930.FOXA1.CFPAC-1 581 bp overlap
ChIP CFPAC-1_FOXA1-KO GSE119930.FOXA1.CFPAC-1_FOXA1-KO 536 bp overlap
ChIP CFPAC-1_FOXA2-KO GSE119930.FOXA1.CFPAC-1_FOXA2-KO 652 bp overlap
ChIP LS180 GSE140533.FOXA1.LS180 559 bp overlap
ChIP PDAC GSE64557.FOXA1.PDAC 607 bp overlap
ChIP PDAC_KOKLF5 GSE64557.FOXA1.PDAC_KOKLF5 711 bp overlap
ChIP prostate-cancer_PDX_136 GSE130408.FOXA1.prostate-cancer_PDX_136 205 bp overlap
FOXA2 8 datasets
ChIP CFPAC-1 GSE119930.FOXA2.CFPAC-1 443 bp overlap
ChIP CFPAC-1_EV GSE119930.FOXA2.CFPAC-1_EV 585 bp overlap
ChIP CFPAC-1_HNF1B-KO GSE119930.FOXA2.CFPAC-1_HNF1B-KO 418 bp overlap
ChIP CFPAC-1_HOXB8-OE GSE119930.FOXA2.CFPAC-1_HOXB8-OE 487 bp overlap
ChIP Caco-2 GSE66218.FOXA2.Caco-2 197 bp overlap
ChIP DE DE-FOXA2-1 334 bp overlap
ChIP DE DE-FOXA2-2 375 bp overlap
ChIP colorectal-cancer_type-C GSE106921.FOXA2.colorectal-cancer_type-C 378 bp overlap
FOXL2 3 datasets
ChIP HGrC1_C134W-TGF GSE138496.FOXL2.HGrC1_C134W-TGF 323 bp overlap
ChIP HGrC1_C134W-TGF_parental GSE138496.FOXL2.HGrC1_C134W-TGF_parental 172 bp overlap
ChIP HGrC1_EV GSE138496.FOXL2.HGrC1_EV 186 bp overlap
FOXP1 1 dataset
ChIP Hep-G2 ENCSR369YUK.FOXP1.Hep-G2 211 bp overlap
Foxq1 1 dataset
Motif DE_12h DE_12h-Foxq1_MA0040.2 10 bp overlap
GATA4 5 datasets
ChIP DE DE-GATA4-1 283 bp overlap
ChIP DE DE-GATA4-2 387 bp overlap
ChIP ESO-26 GSE132813.GATA4.ESO-26 378 bp overlap
ChIP KATO-III GSE51705.GATA4.KATO-III 200 bp overlap
ChIP YCC-3 GSE51705.GATA4.YCC-3 264 bp overlap
GATA6 11 datasets
ChIP AGS GSE51705.GATA6.AGS 504 bp overlap
ChIP AGS GSE51936.GATA6.AGS 233 bp overlap
ChIP DE DE-GATA6-1 311 bp overlap
ChIP DE_D1 S09-DE-d1-GATA6-exp1 808 bp overlap
ChIP DE_D1 S14-DE-d1-GATA6-exp1 662 bp overlap
ChIP DE_D1 S41-DE-d1-GATA6-exp2 808 bp overlap
ChIP HUES-8_DE_JNKi GSE109524.GATA6.HUES-8_DE_JNKi 808 bp overlap
ChIP HUES-8_DE_ctrl GSE109524.GATA6.HUES-8_DE_ctrl 808 bp overlap
ChIP KATO-III GSE51705.GATA6.KATO-III 267 bp overlap
ChIP PATU8988 GSE47535.GATA6.PATU8988 286 bp overlap
ChIP YCC-3 GSE51705.GATA6.YCC-3 516 bp overlap
GFI1 2 datasets
Motif DE_12h DE_12h-GFI1_MA0038.3 11 bp overlap
ChIP NB4 GSE128528.GFI1.NB4 191 bp overlap
Gfi1B 1 dataset
Motif DE_12h DE_12h-Gfi1B_MA0483.2 10 bp overlap
HLF 4 datasets
Motif DE_12h DE_12h-HLF_MA0043.4 9 bp overlap
ChIP Hep-G2 ENCSR528PSI.HLF.Hep-G2 166 bp overlap
ChIP Hep-G2 ENCSR528PSI.HLF.Hep-G2 174 bp overlap
ChIP HepG2 ENCFF854JLR 196 bp overlap
HNF1B 1 dataset
ChIP PDAC GSE64557.HNF1B.PDAC 460 bp overlap
HNF4A 4 datasets
ChIP HCT-116_TCF4 GSE62890.HNF4A.HCT-116_TCF4 224 bp overlap
ChIP HCT-116_TCF4 GSE62890.HNF4A.HCT-116_TCF4 259 bp overlap
ChIP HCT-116_TCF4_DOX GSE62890.HNF4A.HCT-116_TCF4_DOX 507 bp overlap
ChIP KATO-III GSE114018.HNF4A.KATO-III 269 bp overlap
HOXA9 1 dataset
ChIP SEM GSE38339.HOXA9.SEM 190 bp overlap
HOXB8 1 dataset
ChIP CFPAC-1_HOXB8-OE GSE119930.HOXB8.CFPAC-1_HOXB8-OE 462 bp overlap
Hnf1A 2 datasets
Motif DE_12h DE_12h-Hnf1A_MA1991.2 10 bp overlap
Motif DE_12h DE_12h-Hnf1A_MA1991.2 10 bp overlap
ISL2 2 datasets
Motif DE_12h DE_12h-ISL2_MA0914.2 6 bp overlap
ChIP Hep-G2 ENCSR492IHH.ISL2.Hep-G2 195 bp overlap
JARID2 1 dataset
ChIP MRC-5_IPS25_CTR GSE48515.JARID2.MRC-5_IPS25_CTR 357 bp overlap
JUN 3 datasets
ChIP DE_D1 S08-DE-d1-JUN-exp1 623 bp overlap
ChIP DE_D1 S40-DE-d1-JUN-exp2 271 bp overlap
ChIP HUES-8_DE_ctrl GSE109524.JUN.HUES-8_DE_ctrl 657 bp overlap
JUND 1 dataset
ChIP HCT-116 ENCSR000BSA.JUND.HCT-116 134 bp overlap
KDM1A 1 dataset
ChIP SU-DHL-4 GSE119038.KDM1A.SU-DHL-4 220 bp overlap
KLF3 1 dataset
ChIP HEK293 GSE69739.KLF3.HEK293 170 bp overlap
Lef1 2 datasets
Motif DE_12h DE_12h-Lef1_MA0768.3 8 bp overlap
Motif DE_12h DE_12h-Lef1_MA0768.3 8 bp overlap
MED1 1 dataset
ChIP MM1-S_JQ1_5NM GSE49224.MED1.MM1-S_JQ1_5NM 357 bp overlap
MEF2A 3 datasets
Motif DE_12h DE_12h-MEF2A_MA0052.5 10 bp overlap
ChIP GM12878 ENCFF652BHX 221 bp overlap
ChIP GM12878 ENCSR000BKB.MEF2A.GM12878 201 bp overlap
MEF2B 1 dataset
ChIP GM12878 ENCSR177VFS.MEF2B.GM12878 231 bp overlap
MEF2C 3 datasets
Motif DE_12h DE_12h-MEF2C_MA0497.2 11 bp overlap
ChIP GM12878 ENCFF473ASZ 190 bp overlap
ChIP GM12878 ENCSR000BNG.MEF2C.GM12878 124 bp overlap
MEF2D 2 datasets
ChIP SK-UT-1 GSE132622.MEF2D.SK-UT-1 229 bp overlap
ChIP retina_Hu21 GSE137311.MEF2D.retina_Hu21 269 bp overlap
MEIS1 3 datasets
Motif DE_12h DE_12h-MEIS1_MA1639.2 9 bp overlap
ChIP SEM GSE38339.MEIS1.SEM 154 bp overlap
ChIP SEM GSE38339.MEIS1.SEM 160 bp overlap
MEIS2 1 dataset
Motif DE_12h DE_12h-MEIS2_MA1640.2 9 bp overlap
MTA2 1 dataset
ChIP GM12878 ENCSR293QAR.MTA2.GM12878 150 bp overlap
MTA3 1 dataset
ChIP GM12878 ENCSR000BRH.MTA3.GM12878 183 bp overlap
MYB 1 dataset
ChIP DU528 GSE94000.MYB.DU528 683 bp overlap
MYC 3 datasets
ChIP DLD-1_MYC-activated GSE117240.MYC.DLD-1_MYC-activated 154 bp overlap
ChIP GP5D GSE51234.MYC.GP5D 538 bp overlap
ChIP GP5D_SIRAD21 GSE51234.MYC.GP5D_SIRAD21 346 bp overlap
MYCN 1 dataset
ChIP MYCN-3_low GSE83317.MYCN.MYCN-3_low 172 bp overlap
NANOG 1 dataset
ChIP WA01_3IL ERP004238.NANOG.WA01_3IL 174 bp overlap
NCOR1 2 datasets
ChIP LS180_125 GSE39277.NCOR1.LS180_125 101 bp overlap
ChIP LS180_125 GSE39277.NCOR1.LS180_125 101 bp overlap
NFIA 1 dataset
Motif DE_12h DE_12h-NFIA_MA0670.2 6 bp overlap
NFIC 2 datasets
Motif DE_12h DE_12h-NFIC_MA0161.3 7 bp overlap
ChIP GM12878 ENCSR000BRN.NFIC.GM12878 185 bp overlap
NFIL3 1 dataset
Motif DE_12h DE_12h-NFIL3_MA0025.3 9 bp overlap
NFIX 1 dataset
Motif DE_12h DE_12h-NFIX_MA0671.2 6 bp overlap
NIPBL 1 dataset
ChIP GP5D GSE51234.NIPBL.GP5D 569 bp overlap
NKX2-1 1 dataset
ChIP NCI-H3122 GSE39998.NKX2-1.NCI-H3122 166 bp overlap
NR1H2 2 datasets
ChIP HT29_GW3965_2H GSE77039.NR1H2.HT29_GW3965_2H 549 bp overlap
ChIP HT29_GW3965_48H GSE77039.NR1H2.HT29_GW3965_48H 273 bp overlap
NR2F1 1 dataset
ChIP GM12878 ENCSR514VYD.NR2F1.GM12878 229 bp overlap
Nkx3-1 1 dataset
Motif DE_12h DE_12h-Nkx3-1_MA0124.3 7 bp overlap
Nkx3-2 1 dataset
Motif DE_12h DE_12h-Nkx3-2_MA0122.4 10 bp overlap
ONECUT2 2 datasets
ChIP A-549 GSE102599.ONECUT2.A-549 123 bp overlap
ChIP AGS_Overexpression GSE113045.ONECUT2.AGS_Overexpression 296 bp overlap
OSR2 3 datasets
ChIP HEK293 ENCFF875BDB 205 bp overlap
ChIP HEK293 ENCSR324LTM.OSR2.HEK293 623 bp overlap
ChIP HEK293 GSE76494.OSR2.HEK293 156 bp overlap
PAX3-FOXO1 1 dataset
ChIP Hs-352-Sk_PAX3-FOXO1-vector GSE83725.PAX3-FOXO1.Hs-352-Sk_PAX3-FOXO1-vector 183 bp overlap
PAX5 1 dataset
ChIP GM12878 ENCSR000BHD.PAX5.GM12878 116 bp overlap
PAX6 1 dataset
ChIP retina_pigment GSE60024.PAX6.retina_pigment 306 bp overlap
PBX2 1 dataset
Motif DE_12h DE_12h-PBX2_MA1113.3 9 bp overlap
PHOX2B 1 dataset
Motif DE_12h DE_12h-PHOX2B_MA0681.3 12 bp overlap
POU1F1 1 dataset
Motif DE_12h DE_12h-POU1F1_MA0784.3 14 bp overlap
POU2F2 1 dataset
Motif DE_12h DE_12h-POU2F2_MA0507.3 13 bp overlap
POU3F2 1 dataset
Motif DE_12h DE_12h-POU3F2_MA0787.1 12 bp overlap
PPARG 2 datasets
ChIP HT29_ROSIG_2H GSE77039.PPARG.HT29_ROSIG_2H 355 bp overlap
ChIP HT29_ROSIG_48H GSE77039.PPARG.HT29_ROSIG_48H 247 bp overlap
PROX1 2 datasets
ChIP SW480 GSE60390.PROX1.SW480 250 bp overlap
ChIP SW480 GSE60390.PROX1.SW480 98 bp overlap
RAD21 2 datasets
ChIP GP5D GSE51234.RAD21.GP5D 327 bp overlap
ChIP HeLa-Tet-On GSE112028.RAD21.HeLa-Tet-On 700 bp overlap
REST 1 dataset
ChIP colorectal-cancer_shCDH1 GSE112555.REST.colorectal-cancer_shCDH1 282 bp overlap
RNF2 1 dataset
ChIP WA01 ENCSR784VUY.RNF2.WA01 285 bp overlap
RUNX1 2 datasets
ChIP 697 GSE138031.RUNX1.697 194 bp overlap
ChIP Jurkat GSE76181.RUNX1.Jurkat 178 bp overlap
RUNX2 2 datasets
ChIP PER-117 GSE151819.RUNX2.PER-117 202 bp overlap
ChIP PER-117 GSE151819.RUNX2.PER-117 254 bp overlap
RUNX3 1 dataset
ChIP GM12878 ENCFF395WHA 214 bp overlap
RXR 1 dataset
ChIP LS180_125 GSE31939.RXR.LS180_125 144 bp overlap
SKIL 1 dataset
ChIP GM12878 ENCSR212YKD.SKIL.GM12878 154 bp overlap
SMAD2-3 3 datasets
ChIP HGrC1_WT-TGF GSE138496.SMAD2-3.HGrC1_WT-TGF 175 bp overlap
ChIP HUES-8_DE_JNKi GSE109524.SMAD2-3.HUES-8_DE_JNKi 801 bp overlap
ChIP HUES-8_DE_ctrl GSE109524.SMAD2-3.HUES-8_DE_ctrl 808 bp overlap
SMAD2_3 4 datasets
ChIP DE_D1 S07-DE-d1-SMAD2_3-exp1 161 bp overlap
ChIP DE_D1 S07-DE-d1-SMAD2_3-exp1 528 bp overlap
ChIP DE_D1 S12-DE-d1-SMAD2_3-exp1 734 bp overlap
ChIP DE_D1 S39-DE-d1-SMAD2_3-exp2 808 bp overlap
SMARCA4 2 datasets
ChIP hiPSC GSE124903.SMARCA4.hiPSC 199 bp overlap
ChIP hiPSC_WT GSE124903.SMARCA4.hiPSC_WT 166 bp overlap
SMARCB1 3 datasets
ChIP hiPSC GSE124903.SMARCB1.hiPSC 129 bp overlap
ChIP hiPSC_WT GSE124903.SMARCB1.hiPSC_WT 196 bp overlap
ChIP hiPSC_WT GSE124903.SMARCB1.hiPSC_WT 170 bp overlap
SMARCC1 5 datasets
ChIP Aska-SS GSE108025.SMARCC1.Aska-SS 483 bp overlap
ChIP DE_D1 S10-DE-d1-BAF155-exp1 323 bp overlap
ChIP DE_D1 S15-DE-d1-BAF155-exp1 588 bp overlap
ChIP hiPSC GSE124903.SMARCC1.hiPSC 191 bp overlap
ChIP hiPSC_WT GSE124903.SMARCC1.hiPSC_WT 121 bp overlap
SMC3 1 dataset
ChIP GP5D GSE51234.SMC3.GP5D 294 bp overlap
SOX2 1 dataset
ChIP LK2_GFP GSE137459.SOX2.LK2_GFP 621 bp overlap
SOX9 1 dataset
ChIP HT29 GSE63629.SOX9.HT29 309 bp overlap
SP1 2 datasets
ChIP HCT-116 ENCSR000BSF.SP1.HCT-116 184 bp overlap
ChIP HCT116 ENCFF800LBN 349 bp overlap
SP7 1 dataset
ChIP HEK293 ENCSR468IJT.SP7.HEK293 242 bp overlap
SPI1 2 datasets
ChIP CTV-1_delQ GSE128835.SPI1.CTV-1_delQ 189 bp overlap
ChIP CTV-1_delQ GSE128835.SPI1.CTV-1_delQ 216 bp overlap
SS18 1 dataset
ChIP Aska-SS GSE108025.SS18.Aska-SS 475 bp overlap
Spi1 1 dataset
Motif DE_12h DE_12h-Spi1_MA0080.7 13 bp overlap
Spz1 1 dataset
Motif DE_12h DE_12h-Spz1_MA0111.1 11 bp overlap
T 1 dataset
ChIP H9_MESODERM GSE60606.T.H9_MESODERM 225 bp overlap
TBX19 1 dataset
Motif DE_12h DE_12h-TBX19_MA0804.2 17 bp overlap
TBX21 2 datasets
ChIP GM12878 ENCSR739IHN.TBX21.GM12878 220 bp overlap
ChIP Th1_CD3-CD28_donor2 GSE81881.TBX21.Th1_CD3-CD28_donor2 152 bp overlap
TBXT 1 dataset
Motif DE_12h DE_12h-TBXT_MA0009.2 16 bp overlap
TCF4 1 dataset
ChIP LS180_125 GSE31939.TCF4.LS180_125 96 bp overlap
TCF7 4 datasets
Motif DE_12h DE_12h-TCF7_MA0769.3 7 bp overlap
Motif DE_12h DE_12h-TCF7_MA0769.3 7 bp overlap
ChIP GM12878 ENCFF749DPM 142 bp overlap
ChIP GM12878 ENCSR501DKS.TCF7.GM12878 214 bp overlap
TCF7L1 1 dataset
Motif DE_12h DE_12h-TCF7L1_MA1421.1 12 bp overlap
TCF7L2 14 datasets
ChIP CD34_PROG_BIO GSE29194.TCF7L2.CD34_PROG_BIO 122 bp overlap
Motif DE_12h DE_12h-TCF7L2_MA0523.2 9 bp overlap
Motif DE_12h DE_12h-TCF7L2_MA0523.2 9 bp overlap
ChIP HCT-116 ENCSR000EUV.TCF7L2.HCT-116 242 bp overlap
ChIP HCT-116_C16 GSE127960.TCF7L2.HCT-116_C16 260 bp overlap
ChIP HCT-116_C18 GSE127960.TCF7L2.HCT-116_C18 268 bp overlap
ChIP HCT-116_WT GSE127960.TCF7L2.HCT-116_WT 219 bp overlap
ChIP HCT-116_sc2 GSE127960.TCF7L2.HCT-116_sc2 274 bp overlap
ChIP HCT116 ENCFF038POZ 173 bp overlap
ChIP HEK293 ENCFF513JQN 371 bp overlap
ChIP HEK293 ENCSR000EUY.TCF7L2.HEK293 545 bp overlap
ChIP HepG2 ENCFF125ABE 585 bp overlap
ChIP HepG2 ENCFF510OLG 451 bp overlap
ChIP LNCaP GSE51621.TCF7L2.LNCaP 594 bp overlap
TEAD1 1 dataset
ChIP HUCCT1 GSE68296.TEAD1.HUCCT1 167 bp overlap
TEF 1 dataset
Motif DE_12h DE_12h-TEF_MA0843.2 10 bp overlap
TFE3 1 dataset
ChIP Hep-G2 ENCSR589SNT.TFE3.Hep-G2 204 bp overlap
THRB 1 dataset
ChIP Hep-G2 ENCSR430JGJ.THRB.Hep-G2 171 bp overlap
TP63 1 dataset
ChIP BxPC-3 GSE115461.TP63.BxPC-3 374 bp overlap
ZNF189 2 datasets
Motif DE_12h DE_12h-ZNF189_MA1725.2 9 bp overlap
ChIP HEK293 ENCSR163RYW.ZNF189.HEK293 294 bp overlap
ZNF354A 1 dataset
Motif DE_12h DE_12h-ZNF354A_MA1978.2 20 bp overlap