PHOX2B
paired like homeobox 2B | NBPhox, PMX2B

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome. [provided by RefSeq, Jul 2016]

Biological processes 63 terms
DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)autonomic nervous system development (GO:0048483)brainstem development (GO:0003360)cell differentiation in hindbrain (GO:0021533)cellular response to BMP stimulus (GO:0071773)chromatin (GO:0000785)chromatin (GO:0000785)dopaminergic neuron differentiation (GO:0071542)dopaminergic neuron differentiation (GO:0071542)dopaminergic neuron differentiation (GO:0071542)efferent axon development in a lateral line nerve (GO:0048894)enteric nervous system development (GO:0048484)enteric nervous system development (GO:0048484)enteric nervous system development (GO:0048484)glial cell differentiation (GO:0010001)hindbrain tangential cell migration (GO:0021934)inner ear development (GO:0048839)medullary reticular formation development (GO:0021723)medullary reticular formation development (GO:0021723)neural crest cell migration involved in autonomic nervous system development (GO:1901166)neural crest cell migration involved in autonomic nervous system development (GO:1901166)neuron development (GO:0048666)neuron migration (GO:0001764)noradrenergic neuron development (GO:0003358)noradrenergic neuron development (GO:0003358)noradrenergic neuron differentiation (GO:0003357)noradrenergic neuron differentiation (GO:0003357)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)parasympathetic nervous system development (GO:0048486)parasympathetic nervous system development (GO:0048486)positive regulation of G2/M transition of mitotic cell cycle (GO:0010971)positive regulation of cold-induced thermogenesis (GO:0120162)positive regulation of cold-induced thermogenesis (GO:0120162)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)regulation of DNA-templated transcription (GO:0006355)regulation of gene expression (GO:0010468)regulation of gene expression (GO:0010468)regulation of respiratory gaseous exchange by nervous system process (GO:0002087)regulation of respiratory gaseous exchange by nervous system process (GO:0002087)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)respiratory system development (GO:0060541)respiratory system development (GO:0060541)retrotrapezoid nucleus neuron differentiation (GO:0061452)retrotrapezoid nucleus neuron differentiation (GO:0061452)sequence-specific double-stranded DNA binding (GO:1990837)sequence-specific double-stranded DNA binding (GO:1990837)sympathetic ganglion development (GO:0061549)sympathetic ganglion development (GO:0061549)sympathetic nervous system development (GO:0048485)sympathetic nervous system development (GO:0048485)
Expression (TPM)
PHOX2B — as a Regulated Gene

TFs regulating PHOX2B 0 TFs

Transcription factors with Perturb-seq knockdown data for PHOX2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PHOX2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PHOX2B

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PHOX2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr4:41,745,423–41,746,449 1.3 kb Proximal (<10kb) 127
chr4:41,747,180–41,747,918 at TSS At TSS 47
chr4:41,748,050–41,748,788 341 bp At TSS 60
chr4:41,750,881–41,752,090 3.2 kb Proximal (<10kb) 140

Genome Browser

Genomic view of the PHOX2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr4:41,735,423 – 41,762,090
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq