The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RAD21. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RAD21 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RAD21, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:116,669,827–116,670,987 | 204.3 kb | Distal (>10kb) Multiome | 119 | |
| chr8:116,765,869–116,767,075 | 108.3 kb | Distal (>10kb) Multiome | 896 | |
| chr8:116,873,504–116,875,321 | 1.0 kb | Proximal (<10kb) Multiome | 761 | |
| chr8:116,937,903–116,938,912 | 63.7 kb | Distal (>10kb) Multiome | 382 | |
| chr8:117,171,777–117,172,741 | 297.6 kb | Distal (>10kb) Multiome | 183 |
Genomic view of the RAD21 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.