This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
Transcription factors with Perturb-seq knockdown data for CRY2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRY2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRY2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:45,592,587–45,593,123 | 254.7 kb | Distal (>10kb) Multiome | 44 | |
| chr11:45,655,292–45,655,791 | 192.0 kb | Distal (>10kb) Multiome | 271 | |
| chr11:45,664,518–45,666,069 | 181.9 kb | Distal (>10kb) Multiome | 223 | |
| chr11:45,771,302–45,772,261 | 75.5 kb | Distal (>10kb) Multiome | 510 | |
| chr11:45,803,866–45,805,789 | 42.4 kb | Distal (>10kb) Multiome | 806 | |
| chr11:45,843,183–45,843,603 | 3.9 kb | Proximal (<10kb) | 478 | |
| chr11:45,847,011–45,848,213 | 87 bp | At TSS Multiome | 845 | |
| chr11:45,885,234–45,886,606 | 38.2 kb | Distal (>10kb) Multiome | 501 | |
| chr11:45,899,383–45,900,756 | 52.5 kb | Distal (>10kb) Multiome | 534 | |
| chr11:45,917,590–45,918,966 | 71.4 kb | Distal (>10kb) Multiome | 781 | |
| chr11:45,921,513–45,923,586 | 75.0 kb | Distal (>10kb) Multiome | 624 | |
| chr11:46,120,416–46,122,472 | 274.1 kb | Distal (>10kb) Multiome | 758 |
Genomic view of the CRY2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.