Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RNF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RNF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RNF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:184,754,064–184,755,736 | 290.5 kb | Distal (>10kb) Multiome | 971 | |
| chr1:184,905,067–184,906,277 | 139.8 kb | Distal (>10kb) Multiome | 314 | |
| chr1:184,973,585–184,974,868 | 71.3 kb | Distal (>10kb) Multiome | 731 | |
| chr1:185,044,828–185,046,417 | 123 bp | At TSS Multiome | 816 | |
| chr1:185,156,218–185,158,419 | 111.6 kb | Distal (>10kb) Multiome | 949 | |
| chr1:185,315,737–185,318,408 | 272.0 kb | Distal (>10kb) Multiome | 1078 |
Genomic view of the RNF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.