The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
Transcription factors with Perturb-seq knockdown data for NR1H2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NR1H2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NR1H2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:50,147,523–50,148,432 | 228.3 kb | Distal (>10kb) Multiome | 615 | |
| chr19:50,203,034–50,204,072 | 172.9 kb | Distal (>10kb) Multiome | 363 | |
| chr19:50,205,195–50,205,903 | 171.1 kb | Distal (>10kb) Multiome | 364 | |
| chr19:50,311,942–50,312,908 | 64.2 kb | Distal (>10kb) Multiome | 364 | |
| chr19:50,313,900–50,314,529 | 62.2 kb | Distal (>10kb) Multiome | 114 | |
| chr19:50,315,313–50,316,408 | 60.5 kb | Distal (>10kb) Multiome | 363 | |
| chr19:50,325,322–50,326,594 | 50.7 kb | Distal (>10kb) Multiome | 748 | |
| chr19:50,328,019–50,330,066 | 47.1 kb | Distal (>10kb) Multiome | 699 | |
| chr19:50,330,341–50,331,411 | 45.6 kb | Distal (>10kb) Multiome | 479 | |
| chr19:50,332,395–50,334,325 | 42.9 kb | Distal (>10kb) Multiome | 907 | |
| chr19:50,368,630–50,369,092 | 7.5 kb | Proximal (<10kb) Multiome | 311 | |
| chr19:50,369,243–50,369,455 | 7.0 kb | Proximal (<10kb) | 332 | |
| chr19:50,376,225–50,377,452 | 170 bp | At TSS Multiome | 931 | |
| chr19:50,383,444–50,384,805 | 7.8 kb | Proximal (<10kb) Multiome | 863 | |
| chr19:50,414,638–50,415,204 | 38.5 kb | Distal (>10kb) Multiome | 377 | |
| chr19:50,431,430–50,432,503 | 55.4 kb | Distal (>10kb) Multiome | 530 | |
| chr19:50,475,914–50,477,044 | 100.1 kb | Distal (>10kb) Multiome | 622 | |
| chr19:50,510,707–50,511,948 | 134.9 kb | Distal (>10kb) Multiome | 794 | |
| chr19:50,556,266–50,557,084 | 180.2 kb | Distal (>10kb) Multiome | 463 | |
| chr19:50,639,246–50,640,210 | 263.2 kb | Distal (>10kb) Multiome | 326 | |
| chr19:50,657,814–50,659,557 | 281.8 kb | Distal (>10kb) Multiome | 790 |
Genomic view of the NR1H2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.