Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]
Transcription factors with Perturb-seq knockdown data for ZNF189. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF189 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF189, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:100,637,622–100,638,453 | 760.9 kb | Distal (>10kb) Multiome HiCAR | 112 | |
| chr9:101,125,125–101,125,610 | 273.5 kb | Distal (>10kb) Multiome | 73 | |
| chr9:101,134,832–101,136,539 | 262.8 kb | Distal (>10kb) Multiome | 150 | |
| chr9:101,397,915–101,399,348 | 144 bp | At TSS Multiome | 861 | |
| chr9:101,449,087–101,449,796 | 50.5 kb | Distal (>10kb) Multiome | 494 | |
| chr9:101,458,960–101,459,540 | 60.3 kb | Distal (>10kb) Multiome | 253 | |
| chr9:101,460,651–101,461,628 | 62.3 kb | Distal (>10kb) Multiome | 103 | |
| chr9:101,485,920–101,487,719 | 88.1 kb | Distal (>10kb) Multiome | 692 | |
| chr9:101,533,168–101,534,447 | 134.9 kb | Distal (>10kb) Multiome | 723 |
Genomic view of the ZNF189 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.