Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in axonogenesis; neuron fate specification; and positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within negative regulation of neuron differentiation and neuron differentiation. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ISL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ISL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ISL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:76,330,375–76,330,597 | 6.2 kb | Proximal (<10kb) | 140 | |
| chr15:76,333,950–76,334,254 | 2.5 kb | Proximal (<10kb) | 330 | |
| chr15:76,335,050–76,335,692 | 1.1 kb | Proximal (<10kb) | 187 | |
| chr15:76,335,834–76,337,186 | at TSS | At TSS | 781 | |
| chr15:76,337,664–76,338,596 | 892 bp | At TSS | 603 | |
| chr15:76,339,679–76,340,052 | 2.9 kb | Proximal (<10kb) | 92 | |
| chr15:76,340,219–76,340,942 | 3.4 kb | Proximal (<10kb) | 139 | |
| chr15:76,341,048–76,343,196 | 4.3 kb | Proximal (<10kb) | 257 | |
| chr15:76,345,613–76,346,889 | 8.8 kb | Proximal (<10kb) | 577 |
Genomic view of the ISL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.