chr12:121,280,329–121,281,482

chr12 : 121,280,329 – 121,281,482

1,153 bp 112 TFs 7 linked genes

This 1.2 kb open chromatin element is linked to 7 target genes and is bound by 112 transcription factors.

Linked Genes

7 genes

Gene	Expression	Dist. to TSS	Distance	Link type
CAMKK2		15.9 kb	Distal	Multiome+HiCAR
P2RX4		70.7 kb	Distal	Multiome
ANAPC5		71.6 kb	Distal	Multiome
RNF34		119.3 kb	Distal	Multiome
C12orf43		264.4 kb	Distal	Multiome
KDM2B-DT		299.9 kb	Distal	Multiome
KDM2B		300.2 kb	Distal	Multiome

Chromatin Accessibility

Genome Browser

Genomic view centred on this element. Tracks include ATAC-seq accessibility, RNA-seq expression, TF binding peaks, and element-to-gene loop arcs across the ESC → DE time course.

Transcription factors with ChIP-seq peak overlap or TOBIAS motif footprint evidence at this element.

TF Binding

112 transcription factors

Arid3a 1 dataset

Motif DE_24h DE_24h-Arid3a_MA0151.1 6 bp overlap

BCL11A 4 datasets

Motif DE_12h DE_12h-BCL11A_MA2324.1 7 bp overlap

Motif DE_24h DE_24h-BCL11A_MA2324.1 7 bp overlap

Motif DE_36h DE_36h-BCL11A_MA2324.1 7 bp overlap

Motif DE_48h DE_48h-BCL11A_MA2324.1 7 bp overlap

CDX1 3 datasets

Motif DE_24h DE_24h-CDX1_MA0878.3 10 bp overlap

Motif DE_36h DE_36h-CDX1_MA0878.3 10 bp overlap

Motif DE_48h DE_48h-CDX1_MA0878.3 10 bp overlap

Motif DE_24h DE_24h-CTCF_MA1929.2 31 bp overlap

Cebpa 1 dataset

ChIP BLaER1 ENCFF274GAT 279 bp overlap

E2F6 4 datasets

Motif DE_12h DE_12h-E2F6_MA0471.3 8 bp overlap

Motif DE_24h DE_24h-E2F6_MA0471.3 8 bp overlap

Motif DE_36h DE_36h-E2F6_MA0471.3 8 bp overlap

Motif DE_48h DE_48h-E2F6_MA0471.3 8 bp overlap

Motif DE_24h DE_24h-EGR4_MA0733.2 11 bp overlap

ERF::FIGLA 4 datasets

Motif DE_24h DE_24h-ERFFIGLA_MA1934.2 13 bp overlap

Motif DE_24h DE_24h-ERFFIGLA_MA1934.2 13 bp overlap

Motif DE_24h DE_24h-ERFFIGLA_MA1934.2 13 bp overlap

Motif DE_24h DE_24h-ERFFIGLA_MA1934.2 13 bp overlap

ESR2 3 datasets

Motif DE_24h DE_24h-ESR2_MA0258.2 15 bp overlap

Motif DE_36h DE_36h-ESR2_MA0258.2 15 bp overlap

Motif DE_48h DE_48h-ESR2_MA0258.2 15 bp overlap

ETV2::FIGLA 3 datasets

Motif DE_24h DE_24h-ETV2FIGLA_MA1941.2 13 bp overlap

Motif DE_24h DE_24h-ETV2FIGLA_MA1941.2 13 bp overlap

Motif DE_24h DE_24h-ETV2FIGLA_MA1941.2 13 bp overlap

ETV5::FIGLA 3 datasets

Motif DE_24h DE_24h-ETV5FIGLA_MA1945.2 14 bp overlap

Motif DE_24h DE_24h-ETV5FIGLA_MA1945.2 14 bp overlap

Motif DE_24h DE_24h-ETV5FIGLA_MA1945.2 14 bp overlap

ETV5::FOXO1 3 datasets

Motif DE_24h DE_24h-ETV5FOXO1_MA1947.2 10 bp overlap

Motif DE_36h DE_36h-ETV5FOXO1_MA1947.2 10 bp overlap

Motif DE_48h DE_48h-ETV5FOXO1_MA1947.2 10 bp overlap

FEZF2 3 datasets

Motif DE_24h DE_24h-FEZF2_MA2341.1 8 bp overlap

Motif DE_36h DE_36h-FEZF2_MA2341.1 8 bp overlap

Motif DE_48h DE_48h-FEZF2_MA2341.1 8 bp overlap

FIGLA 5 datasets

Motif DE_12h DE_12h-FIGLA_MA0820.2 6 bp overlap

Motif DE_24h DE_24h-FIGLA_MA0820.2 6 bp overlap

Motif DE_24h DE_24h-FIGLA_MA0820.2 6 bp overlap

Motif DE_36h DE_36h-FIGLA_MA0820.2 6 bp overlap

Motif DE_48h DE_48h-FIGLA_MA0820.2 6 bp overlap

FOXA2 2 datasets

ChIP DE DE-FOXA2-1 442 bp overlap

ChIP DE DE-FOXA2-2 393 bp overlap

FOXD3 1 dataset

Motif DE_24h DE_24h-FOXD3_MA0041.3 14 bp overlap

GATA4 4 datasets

ChIP DE DE-GATA4-1 874 bp overlap

ChIP DE DE-GATA4-2 916 bp overlap

Motif DE_24h DE_24h-GATA4_MA0482.3 8 bp overlap

Motif DE_36h DE_36h-GATA4_MA0482.3 8 bp overlap

GATA5 2 datasets

Motif DE_24h DE_24h-GATA5_MA0766.3 8 bp overlap

Motif DE_36h DE_36h-GATA5_MA0766.3 8 bp overlap

GATA6 10 datasets

ChIP DE DE-GATA6-1 909 bp overlap

ChIP DE DE-GATA6-2 1079 bp overlap

Motif DE_24h DE_24h-GATA6_MA1104.3 8 bp overlap

Motif DE_36h DE_36h-GATA6_MA1104.3 8 bp overlap

ChIP DE_D1 S09-DE-d1-GATA6-exp1 766 bp overlap

ChIP DE_D1 S14-DE-d1-GATA6-exp1 919 bp overlap

ChIP DE_D1 S41-DE-d1-GATA6-exp2 1052 bp overlap

ChIP DE_D2 S55-DE-d2-GATA6-exp2 754 bp overlap

ChIP HUES-8_DE_JNKi GSE109524.GATA6.HUES-8_DE_JNKi 476 bp overlap

ChIP HUES-8_DE_ctrl GSE109524.GATA6.HUES-8_DE_ctrl 360 bp overlap

GLIS1 1 dataset

Motif DE_24h DE_24h-GLIS1_MA0735.2 15 bp overlap

GLIS2 2 datasets

Motif DE_24h DE_24h-GLIS2_MA0736.1 14 bp overlap

Motif DE_24h DE_24h-GLIS2_MA0736.1 14 bp overlap

GLIS3 2 datasets

Motif DE_24h DE_24h-GLIS3_MA0737.1 14 bp overlap

Motif DE_24h DE_24h-GLIS3_MA0737.1 14 bp overlap

Gata3 2 datasets

Motif DE_24h DE_24h-Gata3_MA0037.5 8 bp overlap

Motif DE_36h DE_36h-Gata3_MA0037.5 8 bp overlap

HOXB4 1 dataset

Motif DE_24h DE_24h-HOXB4_MA1499.2 6 bp overlap

HOXC4 1 dataset

Motif DE_24h DE_24h-HOXC4_MA1504.2 6 bp overlap

HOXD4 1 dataset

Motif DE_24h DE_24h-HOXD4_MA1507.2 6 bp overlap

HSF1 2 datasets

Motif DE_24h DE_24h-HSF1_MA0486.2 13 bp overlap

Motif DE_36h DE_36h-HSF1_MA0486.2 13 bp overlap

HSF4 2 datasets

Motif DE_24h DE_24h-HSF4_MA0771.1 13 bp overlap

Motif DE_36h DE_36h-HSF4_MA0771.1 13 bp overlap

Hmga1 1 dataset

Motif DE_24h DE_24h-Hmga1_MA2124.1 8 bp overlap

Hnf1A 3 datasets

Motif DE_24h DE_24h-Hnf1A_MA1991.2 10 bp overlap

Motif DE_36h DE_36h-Hnf1A_MA1991.2 10 bp overlap

Motif DE_48h DE_48h-Hnf1A_MA1991.2 10 bp overlap

IKZF2 4 datasets

Motif DE_12h DE_12h-IKZF2_MA2326.1 6 bp overlap

Motif DE_24h DE_24h-IKZF2_MA2326.1 6 bp overlap

Motif DE_36h DE_36h-IKZF2_MA2326.1 6 bp overlap

Motif DE_48h DE_48h-IKZF2_MA2326.1 6 bp overlap

IRF6 2 datasets

Motif DE_24h DE_24h-IRF6_MA1509.1 9 bp overlap

Motif DE_36h DE_36h-IRF6_MA1509.1 9 bp overlap

Motif DE_24h DE_24h-Irf1_MA0050.4 11 bp overlap

ChIP DE_D1 S08-DE-d1-JUN-exp1 508 bp overlap

ChIP DE_D1 S13-DE-d1-JUN-exp1 264 bp overlap

ChIP DE_D1 S40-DE-d1-JUN-exp2 641 bp overlap

ChIP DE_D2 S02-DE-d2-JUN-exp1 617 bp overlap

JUND 3 datasets

Motif DE_24h DE_24h-JUND_MA0492.2 11 bp overlap

Motif DE_36h DE_36h-JUND_MA0492.2 11 bp overlap

Motif DE_48h DE_48h-JUND_MA0492.2 11 bp overlap

MEIS1 3 datasets

Motif DE_24h DE_24h-MEIS1_MA0498.3 5 bp overlap

Motif DE_24h DE_24h-MEIS1_MA0498.3 5 bp overlap

Motif DE_36h DE_36h-MEIS1_MA0498.3 5 bp overlap

MGA::EVX1 4 datasets

Motif DE_12h DE_12h-MGAEVX1_MA1960.2 11 bp overlap

Motif DE_24h DE_24h-MGAEVX1_MA1960.2 11 bp overlap

Motif DE_36h DE_36h-MGAEVX1_MA1960.2 11 bp overlap

Motif DE_48h DE_48h-MGAEVX1_MA1960.2 11 bp overlap

NFATC3 1 dataset

Motif DE_24h DE_24h-NFATC3_MA0625.3 6 bp overlap

NFATC4 1 dataset

Motif DE_24h DE_24h-NFATC4_MA1525.3 9 bp overlap

Motif DE_24h DE_24h-NFIA_MA0670.2 6 bp overlap

Motif DE_24h DE_24h-NFIX_MA0671.2 6 bp overlap

NFKB2 4 datasets

Motif DE_12h DE_12h-NFKB2_MA0778.2 11 bp overlap

Motif DE_24h DE_24h-NFKB2_MA0778.2 11 bp overlap

Motif DE_36h DE_36h-NFKB2_MA0778.2 11 bp overlap

Motif DE_48h DE_48h-NFKB2_MA0778.2 11 bp overlap

NR1H4::RXRA 1 dataset

Motif DE_24h DE_24h-NR1H4RXRA_MA1146.2 13 bp overlap

NR2C2 3 datasets

Motif DE_24h DE_24h-NR2C2_MA0504.2 14 bp overlap

Motif DE_36h DE_36h-NR2C2_MA0504.2 14 bp overlap

Motif DE_48h DE_48h-NR2C2_MA0504.2 14 bp overlap

NR2F6 1 dataset

Motif DE_24h DE_24h-NR2F6_MA1539.1 15 bp overlap

NR4A2::RXRA 1 dataset

Motif DE_24h DE_24h-NR4A2RXRA_MA1147.2 13 bp overlap

NR6A1 1 dataset

Motif DE_24h DE_24h-NR6A1_MA1541.2 14 bp overlap

Nfat5 1 dataset

Motif DE_24h DE_24h-Nfat5_MA0606.3 8 bp overlap

Nfatc1 1 dataset

Motif DE_24h DE_24h-Nfatc1_MA0624.3 6 bp overlap

Motif DE_24h DE_24h-PBX3_MA1114.2 11 bp overlap

PHOX2A 1 dataset

Motif DE_24h DE_24h-PHOX2A_MA0713.1 11 bp overlap

PKNOX1 1 dataset

Motif DE_24h DE_24h-PKNOX1_MA0782.3 10 bp overlap

POU1F1 1 dataset

Motif DE_24h DE_24h-POU1F1_MA0784.3 14 bp overlap

POU2F1::SOX2 2 datasets

Motif DE_24h DE_24h-POU2F1SOX2_MA1962.1 17 bp overlap

Motif DE_36h DE_36h-POU2F1SOX2_MA1962.1 17 bp overlap

POU5F1 1 dataset

ChIP DE_D1 DED1-OCT4_Batch_II 968 bp overlap

POU5F1_M 1 dataset

ChIP DE_D1 DED1-OCT4-M_Batch_II 919 bp overlap

POU6F2 1 dataset

Motif DE_24h DE_24h-POU6F2_MA0793.2 9 bp overlap

PRDM1 1 dataset

Motif DE_24h DE_24h-PRDM1_MA0508.4 7 bp overlap

Pou5f1::Sox2 2 datasets

Motif DE_24h DE_24h-Pou5f1Sox2_MA0142.1 15 bp overlap

Motif DE_36h DE_36h-Pou5f1Sox2_MA0142.1 15 bp overlap

RBPJ 4 datasets

Motif DE_12h DE_12h-RBPJ_MA1116.2 6 bp overlap

Motif DE_24h DE_24h-RBPJ_MA1116.2 6 bp overlap

Motif DE_36h DE_36h-RBPJ_MA1116.2 6 bp overlap

Motif DE_48h DE_48h-RBPJ_MA1116.2 6 bp overlap

Motif DE_12h DE_12h-REL_MA0101.1 10 bp overlap

Motif DE_24h DE_24h-REL_MA0101.1 10 bp overlap

Motif DE_36h DE_36h-REL_MA0101.1 10 bp overlap

Motif DE_48h DE_48h-REL_MA0101.1 10 bp overlap

RELA 4 datasets

Motif DE_12h DE_12h-RELA_MA0107.1 10 bp overlap

Motif DE_24h DE_24h-RELA_MA0107.1 10 bp overlap

Motif DE_36h DE_36h-RELA_MA0107.1 10 bp overlap

Motif DE_48h DE_48h-RELA_MA0107.1 10 bp overlap

RORA 4 datasets

Motif DE_12h DE_12h-RORA_MA0072.2 11 bp overlap

Motif DE_24h DE_24h-RORA_MA0072.2 11 bp overlap

Motif DE_36h DE_36h-RORA_MA0072.2 11 bp overlap

Motif DE_48h DE_48h-RORA_MA0072.2 11 bp overlap

SATB1 4 datasets

Motif DE_12h DE_12h-SATB1_MA1963.2 7 bp overlap

Motif DE_24h DE_24h-SATB1_MA1963.2 7 bp overlap

Motif DE_36h DE_36h-SATB1_MA1963.2 7 bp overlap

Motif DE_48h DE_48h-SATB1_MA1963.2 7 bp overlap

SMAD2-3 3 datasets

ChIP HUES-8_DE GSE109524.SMAD2-3.HUES-8_DE 695 bp overlap

ChIP HUES-8_DE_JNKi GSE109524.SMAD2-3.HUES-8_DE_JNKi 851 bp overlap

ChIP HUES-8_DE_ctrl GSE109524.SMAD2-3.HUES-8_DE_ctrl 539 bp overlap

SMAD2_3 5 datasets

ChIP DE_D1 S07-DE-d1-SMAD2_3-exp1 1090 bp overlap

ChIP DE_D1 S12-DE-d1-SMAD2_3-exp1 1087 bp overlap

ChIP DE_D1 S39-DE-d1-SMAD2_3-exp2 1023 bp overlap

ChIP DE_D2 S01-DE-d2-SMAD2_3-exp1 1153 bp overlap

ChIP DE_D2 S53-DE-d2-SMAD2_3-exp2 996 bp overlap

SMARCC1 2 datasets

ChIP DE_D1 S15-DE-d1-BAF155-exp1 451 bp overlap

ChIP DE_D1 S15-DE-d1-BAF155-exp1 298 bp overlap

SOX17 1 dataset

ChIP DE_D2 DED2-SOX17_Batch_II 372 bp overlap

SOX17_M 1 dataset

ChIP DE_D2 DED2-SOX17-M_Batch_II 972 bp overlap

SOX21 3 datasets

Motif DE_24h DE_24h-SOX21_MA0866.1 15 bp overlap

Motif DE_24h DE_24h-SOX21_MA0866.1 15 bp overlap

Motif DE_36h DE_36h-SOX21_MA0866.1 15 bp overlap

Motif DE_24h DE_24h-SP5_MA1965.2 6 bp overlap

SREBF1 2 datasets

Motif DE_24h DE_24h-SREBF1_MA0829.3 10 bp overlap

Motif DE_36h DE_36h-SREBF1_MA0829.3 10 bp overlap

STAT1 1 dataset

Motif DE_24h DE_24h-STAT1_MA0137.4 9 bp overlap

Six3 3 datasets

Motif DE_24h DE_24h-Six3_MA0631.2 11 bp overlap

Motif DE_36h DE_36h-Six3_MA0631.2 11 bp overlap

Motif DE_48h DE_48h-Six3_MA0631.2 11 bp overlap

Sox1 2 datasets

Motif DE_24h DE_24h-Sox1_MA0870.1 15 bp overlap

Motif DE_36h DE_36h-Sox1_MA0870.1 15 bp overlap

Stat2 2 datasets

Motif DE_24h DE_24h-Stat2_MA1623.2 10 bp overlap

Motif DE_24h DE_24h-Stat2_MA1623.2 10 bp overlap

Stat5a 1 dataset

Motif DE_24h DE_24h-Stat5a_MA1624.2 9 bp overlap

TBX18 4 datasets

Motif DE_12h DE_12h-TBX18_MA1565.2 9 bp overlap

Motif DE_24h DE_24h-TBX18_MA1565.2 9 bp overlap

Motif DE_36h DE_36h-TBX18_MA1565.2 9 bp overlap

Motif DE_48h DE_48h-TBX18_MA1565.2 9 bp overlap

Motif DE_24h DE_24h-TBX5_MA0807.1 8 bp overlap

TFAP2A 2 datasets

Motif DE_24h DE_24h-TFAP2A_MA0810.2 11 bp overlap

Motif DE_24h DE_24h-TFAP2A_MA0872.1 13 bp overlap

TFAP2B 2 datasets

Motif DE_24h DE_24h-TFAP2B_MA0811.2 11 bp overlap

Motif DE_24h DE_24h-TFAP2B_MA0813.1 13 bp overlap

TFAP2C 1 dataset

Motif DE_24h DE_24h-TFAP2C_MA0815.1 13 bp overlap

TFAP4::ETV1 3 datasets

Motif DE_24h DE_24h-TFAP4ETV1_MA1966.2 13 bp overlap

Motif DE_24h DE_24h-TFAP4ETV1_MA1966.2 13 bp overlap

Motif DE_24h DE_24h-TFAP4ETV1_MA1966.2 13 bp overlap

TFAP4::FLI1 1 dataset

Motif DE_24h DE_24h-TFAP4FLI1_MA1967.2 14 bp overlap

TFCP2 4 datasets

Motif DE_12h DE_12h-TFCP2_MA1968.2 9 bp overlap

Motif DE_24h DE_24h-TFCP2_MA1968.2 9 bp overlap

Motif DE_36h DE_36h-TFCP2_MA1968.2 9 bp overlap

Motif DE_48h DE_48h-TFCP2_MA1968.2 9 bp overlap

TGIF1 1 dataset

Motif DE_24h DE_24h-TGIF1_MA0796.1 12 bp overlap

TGIF2 1 dataset

Motif DE_24h DE_24h-TGIF2_MA0797.1 12 bp overlap

TGIF2LX 1 dataset

Motif DE_24h DE_24h-TGIF2LX_MA1571.1 12 bp overlap

TGIF2LY 1 dataset

Motif DE_24h DE_24h-TGIF2LY_MA1572.1 12 bp overlap

TRPS1 2 datasets

Motif DE_24h DE_24h-TRPS1_MA1970.2 8 bp overlap

Motif DE_36h DE_36h-TRPS1_MA1970.2 8 bp overlap

Tbx6 4 datasets

Motif DE_12h DE_12h-Tbx6_MA1567.3 9 bp overlap

Motif DE_24h DE_24h-Tbx6_MA1567.3 9 bp overlap

Motif DE_36h DE_36h-Tbx6_MA1567.3 9 bp overlap

Motif DE_48h DE_48h-Tbx6_MA1567.3 9 bp overlap

Thap11 2 datasets

Motif DE_24h DE_24h-Thap11_MA1573.2 14 bp overlap

Motif DE_36h DE_36h-Thap11_MA1573.2 14 bp overlap

VEZF1 1 dataset

Motif DE_24h DE_24h-VEZF1_MA1578.2 6 bp overlap

ZBTB14 1 dataset

Motif DE_24h DE_24h-ZBTB14_MA1650.2 8 bp overlap

ZBTB24 2 datasets

Motif DE_24h DE_24h-ZBTB24_MA2330.1 10 bp overlap

Motif DE_36h DE_36h-ZBTB24_MA2330.1 10 bp overlap

ZEB1 5 datasets

Motif DE_12h DE_12h-ZEB1_MA0103.4 6 bp overlap

Motif DE_24h DE_24h-ZEB1_MA0103.4 6 bp overlap

Motif DE_24h DE_24h-ZEB1_MA0103.4 6 bp overlap

Motif DE_36h DE_36h-ZEB1_MA0103.4 6 bp overlap

Motif DE_48h DE_48h-ZEB1_MA0103.4 6 bp overlap

Motif DE_24h DE_24h-ZIC1_MA0696.1 14 bp overlap

Motif DE_24h DE_24h-ZIC4_MA0751.2 14 bp overlap

Motif DE_24h DE_24h-ZIC5_MA1584.2 15 bp overlap

Motif DE_24h DE_24h-ZIM3_MA1709.2 11 bp overlap

ZKSCAN5 4 datasets

Motif DE_24h DE_24h-ZKSCAN5_MA1652.2 9 bp overlap

Motif DE_24h DE_24h-ZKSCAN5_MA1652.2 9 bp overlap

Motif DE_36h DE_36h-ZKSCAN5_MA1652.2 9 bp overlap

Motif DE_48h DE_48h-ZKSCAN5_MA1652.2 9 bp overlap

ZNF24 4 datasets

ChIP K-562 ENCSR099NCH.ZNF24.K-562 202 bp overlap

ChIP K-562 ENCSR695EQB.ZNF24.K-562 161 bp overlap

ChIP K562 ENCFF615YYW 171 bp overlap

ChIP K562 ENCFF877JCX 135 bp overlap

ZNF257 6 datasets

Motif DE_24h DE_24h-ZNF257_MA1710.2 10 bp overlap

Motif DE_24h DE_24h-ZNF257_MA1710.2 10 bp overlap

Motif DE_36h DE_36h-ZNF257_MA1710.2 10 bp overlap

Motif DE_36h DE_36h-ZNF257_MA1710.2 10 bp overlap

Motif DE_48h DE_48h-ZNF257_MA1710.2 10 bp overlap

Motif DE_48h DE_48h-ZNF257_MA1710.2 10 bp overlap

ZNF281 3 datasets

Motif DE_24h DE_24h-ZNF281_MA1630.3 10 bp overlap

Motif DE_36h DE_36h-ZNF281_MA1630.3 10 bp overlap

Motif DE_48h DE_48h-ZNF281_MA1630.3 10 bp overlap

ZNF320 3 datasets

Motif DE_24h DE_24h-ZNF320_MA1976.2 20 bp overlap

Motif DE_36h DE_36h-ZNF320_MA1976.2 20 bp overlap

Motif DE_48h DE_48h-ZNF320_MA1976.2 20 bp overlap

ZNF341 1 dataset

Motif DE_24h DE_24h-ZNF341_MA1655.2 8 bp overlap

ZNF354A 1 dataset

Motif DE_24h DE_24h-ZNF354A_MA1978.2 20 bp overlap

ZNF460 1 dataset

Motif DE_24h DE_24h-ZNF460_MA1596.1 16 bp overlap

ZNF547 1 dataset

Motif DE_24h DE_24h-ZNF547_MA2334.1 13 bp overlap

ZNF784 1 dataset

Motif DE_24h DE_24h-ZNF784_MA1717.2 8 bp overlap

ZSCAN4 1 dataset

Motif DE_24h DE_24h-ZSCAN4_MA1155.1 15 bp overlap

Zfp961 2 datasets

Motif DE_24h DE_24h-Zfp961_MA2126.1 8 bp overlap

Motif DE_24h DE_24h-Zfp961_MA2126.1 8 bp overlap