Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of gene expression; negative regulation of transcription by RNA polymerase II; and positive regulation of DNA-templated transcription. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZNF281. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF281 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF281, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:200,129,357–200,130,017 | 280.3 kb | Distal (>10kb) Multiome | 129 | |
| chr1:200,225,879–200,226,951 | 183.6 kb | Distal (>10kb) Multiome | 304 | |
| chr1:200,275,106–200,277,040 | 134.5 kb | Distal (>10kb) Multiome | 305 | |
| chr1:200,364,189–200,365,401 | 45.0 kb | Distal (>10kb) Multiome | 292 | |
| chr1:200,368,023–200,369,613 | 41.4 kb | Distal (>10kb) Multiome | 213 | |
| chr1:200,409,102–200,410,859 | 100 bp | At TSS Multiome | 818 | |
| chr1:200,411,526–200,412,290 | 1.8 kb | Proximal (<10kb) Multiome | 202 | |
| chr1:200,620,328–200,621,096 | 210.8 kb | Distal (>10kb) Multiome | 739 | |
| chr1:200,669,293–200,670,631 | 259.9 kb | Distal (>10kb) Multiome | 1078 |
Genomic view of the ZNF281 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.