SRY-related HMG-box (SOX) genes encode a family of DNA-binding proteins containing a 79-amino acid HMG (high mobility group) domain that shares at least 50% sequence identity with the DNA-binding HMG box of the SRY protein (MIM 480000). SOX proteins are divided into 6 subgroups based on sequence similarity within and outside of the HMG domain. For additional background information on SOX genes, see SOX1 (MIM 602148).[supplied by OMIM, Apr 2004]
Transcription factors with Perturb-seq knockdown data for SOX21. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SOX21 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SOX21, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:94,548,604–94,550,382 | 162.9 kb | Distal (>10kb) Multiome | 899 | |
| chr13:94,595,700–94,596,749 | 116.3 kb | Distal (>10kb) Multiome | 741 | |
| chr13:94,601,312–94,602,589 | 110.7 kb | Distal (>10kb) Multiome | 663 | |
| chr13:94,707,389–94,708,611 | 4.2 kb | Proximal (<10kb) Multiome | 257 | |
| chr13:94,710,520–94,714,056 | 1.2 kb | Proximal (<10kb) Multiome | 498 | |
| chr13:94,717,747–94,718,273 | 5.2 kb | Proximal (<10kb) | 82 | |
| chr13:94,825,487–94,826,097 | 113.2 kb | Distal (>10kb) Multiome | 148 | |
| chr13:94,967,454–94,969,003 | 255.6 kb | Distal (>10kb) Multiome HiCAR | 469 |
Genomic view of the SOX21 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.