The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]
Transcription factors with Perturb-seq knockdown data for RBPJ. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RBPJ upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RBPJ, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:26,028,604–26,030,844 | 291.8 kb | Distal (>10kb) Multiome HiCAR | 374 | |
| chr4:26,238,564–26,239,801 | 81.8 kb | Distal (>10kb) Multiome | 193 | |
| chr4:26,318,840–26,321,924 | 1.2 kb | Proximal (<10kb) Multiome | 1097 | |
| chr4:26,322,043–26,322,235 | 1.3 kb | Proximal (<10kb) | 115 | |
| chr4:26,325,068–26,325,931 | 4.3 kb | Proximal (<10kb) | 113 | |
| chr4:26,330,262–26,330,480 | 9.5 kb | Proximal (<10kb) | 113 | |
| chr4:26,583,577–26,584,548 | 263.3 kb | Distal (>10kb) Multiome | 965 |
Genomic view of the RBPJ locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.