This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]
Transcription factors with Perturb-seq knockdown data for ANAPC5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANAPC5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANAPC5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:121,209,800–121,210,863 | 142.3 kb | Distal (>10kb) Multiome | 894 | |
| chr12:121,230,137–121,230,781 | 121.9 kb | Distal (>10kb) Multiome | 326 | |
| chr12:121,280,329–121,281,482 | 71.6 kb | Distal (>10kb) Multiome | 112 | |
| chr12:121,295,441–121,297,879 | 55.5 kb | Distal (>10kb) Multiome | 801 | |
| chr12:121,351,979–121,352,906 | 67 bp | At TSS Multiome | 928 | |
| chr12:121,399,561–121,400,983 | 47.6 kb | Distal (>10kb) Multiome HiCAR | 923 | |
| chr12:121,443,293–121,443,776 | 91.0 kb | Distal (>10kb) Multiome | 302 | |
| chr12:121,466,702–121,468,225 | 115.0 kb | Distal (>10kb) Multiome | 643 | |
| chr12:121,536,039–121,536,480 | 183.9 kb | Distal (>10kb) Multiome | 262 | |
| chr12:121,576,794–121,577,632 | 224.7 kb | Distal (>10kb) Multiome | 171 | |
| chr12:121,580,160–121,581,909 | 228.0 kb | Distal (>10kb) Multiome | 629 | |
| chr12:121,582,525–121,583,038 | 230.4 kb | Distal (>10kb) Multiome | 309 | |
| chr12:121,625,878–121,627,559 | 274.1 kb | Distal (>10kb) Multiome | 904 |
Genomic view of the ANAPC5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.