This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. The encoded protein may act as a transcriptional repressor. Studies in mouse and Xenopus support a role for this gene in neural crest development. Elevated expression of this gene has been observed in various human cancers and may contribute to cancer progression. This gene is closely linked to a related family member on chromosome 13. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for ZIC5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZIC5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZIC5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr13:99,605,931–99,607,157 | 365.1 kb | Distal (>10kb) Multiome HiCAR | 845 | |
| chr13:99,657,854–99,658,963 | 313.3 kb | Distal (>10kb) Multiome HiCAR | 252 | |
| chr13:99,686,744–99,688,098 | 284.6 kb | Distal (>10kb) Multiome HiCAR | 332 | |
| chr13:99,818,120–99,818,590 | 153.4 kb | Distal (>10kb) Multiome | 93 | |
| chr13:99,955,497–99,958,187 | 14.1 kb | Distal (>10kb) Multiome | 501 | |
| chr13:99,967,588–99,968,838 | 2.9 kb | Proximal (<10kb) | 225 | |
| chr13:99,970,037–99,972,702 | 154 bp | At TSS Multiome | 629 | |
| chr13:99,974,409–99,974,618 | 2.6 kb | Proximal (<10kb) | 53 | |
| chr13:99,974,729–99,975,144 | 3.0 kb | Proximal (<10kb) | 81 | |
| chr13:99,978,424–99,982,746 | 7.4 kb | Proximal (<10kb) Multiome | 812 | |
| chr13:99,990,093–99,992,597 | 20.1 kb | Distal (>10kb) Multiome | 378 | |
| chr13:99,996,965–99,998,156 | 25.7 kb | Distal (>10kb) Multiome | 258 | |
| chr13:100,050,037–100,051,771 | 79.4 kb | Distal (>10kb) Multiome | 289 | |
| chr13:100,088,371–100,089,589 | 117.2 kb | Distal (>10kb) Multiome | 925 | |
| chr13:100,510,174–100,511,130 | 538.7 kb | Distal (>10kb) Multiome HiCAR | 264 | |
| chr13:100,591,344–100,592,134 | 620.0 kb | Distal (>10kb) Multiome HiCAR | 51 |
Genomic view of the ZIC5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.