chr20 : 25,867,953 25,868,741
788 bp 94 TFs 1 linked gene
This 788 bp open chromatin element is linked to FAM182B and is bound by 94 transcription factors.
Linked Genes
1 gene
Gene Expression Dist. to TSS Distance Link type
FAM182B 8.1 kb Proximal Proximity
Chromatin Accessibility

Genome Browser

Genomic view centred on this element. Tracks include ATAC-seq accessibility, RNA-seq expression, TF binding peaks, and element-to-gene loop arcs across the ESC → DE time course.

View limited to ±500 kb around this element.
For the full data, please see the UCSC track linked below.
chr20:25,862,953 – 25,873,741
This element Other elements Gene links ATAC-seq RNA-seq

Transcription factors with ChIP-seq peak overlap or TOBIAS motif footprint evidence at this element.

TF Binding
94 transcription factors
Source
Cell type
AR 2 datasets
ChIP myofibroblast GSE90772.AR.myofibroblast 333 bp overlap
ChIP prostate_cancer-associated-fibroblasts GSE90772.AR.prostate_cancer-associated-fibroblasts 292 bp overlap
ASCL1 3 datasets
Motif DE_48h DE_48h-ASCL1_MA1100.3 8 bp overlap
ChIP NCI-H2107 GSE69394.ASCL1.NCI-H2107 142 bp overlap
ChIP SCLC_ASCLP GSE61197.ASCL1.SCLC_ASCLP 182 bp overlap
Alx4 5 datasets
Motif DE_12h DE_12h-Alx4_MA0853.2 12 bp overlap
Motif DE_36h DE_36h-Alx4_MA0853.2 12 bp overlap
Motif DE_48h DE_48h-Alx4_MA0853.2 12 bp overlap
Motif DE_60h DE_60h-Alx4_MA0853.2 12 bp overlap
Motif DE_72h DE_72h-Alx4_MA0853.2 12 bp overlap
Arx 5 datasets
Motif DE_12h DE_12h-Arx_MA0874.2 10 bp overlap
Motif DE_36h DE_36h-Arx_MA0874.2 10 bp overlap
Motif DE_48h DE_48h-Arx_MA0874.2 10 bp overlap
Motif DE_60h DE_60h-Arx_MA0874.2 10 bp overlap
Motif DE_72h DE_72h-Arx_MA0874.2 10 bp overlap
Ascl2 1 dataset
Motif DE_48h DE_48h-Ascl2_MA0816.1 10 bp overlap
BCL11B 1 dataset
ChIP thymus_CD34neg GSE84677.BCL11B.thymus_CD34neg 134 bp overlap
BCOR 2 datasets
ChIP WA01_RNF2-R GSE104690.BCOR.WA01_RNF2-R 192 bp overlap
ChIP WA01_RNF2-R_Doxycyclin GSE104690.BCOR.WA01_RNF2-R_Doxycyclin 353 bp overlap
BRCA1 2 datasets
ChIP TC-32 GSE87324.BRCA1.TC-32 201 bp overlap
ChIP U2OS GSE87324.BRCA1.U2OS 417 bp overlap
BRD3 5 datasets
ChIP HEK293T GSE39579.BRD3.HEK293T 135 bp overlap
ChIP MM1-S GSE43743.BRD3.MM1-S 405 bp overlap
ChIP U-87MG_GBM GSE99171.BRD3.U-87MG_GBM 294 bp overlap
ChIP U-87MG_GBM_dBET6_1d GSE99171.BRD3.U-87MG_GBM_dBET6_1d 236 bp overlap
ChIP U-87MG_GBM_dBET6_2h GSE99171.BRD3.U-87MG_GBM_dBET6_2h 268 bp overlap
BRD4 3 datasets
ChIP CD4_Th1_BAY GSE62482.BRD4.CD4_Th1_BAY 116 bp overlap
ChIP retina_AB1-FW15 GSE86981.BRD4.retina_AB1-FW15 381 bp overlap
ChIP retina_AB1-RB GSE86981.BRD4.retina_AB1-RB 219 bp overlap
CTCF 26 datasets
ChIP BJ ENCFF434HEC 241 bp overlap
ChIP anterior-temporal-cortex_fetal GSE116825.CTCF.anterior-temporal-cortex_fetal 227 bp overlap
ChIP ascending aorta ENCFF451CCT 325 bp overlap
ChIP astrocyte of the spinal cord ENCFF213GKL 245 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF277CZQ 505 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF403LNW 517 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF433UFM 545 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF458OBF 511 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF483ZLP 501 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF595WAL 491 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF696JOF 505 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF748IBN 517 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF749FBO 477 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF749FBO 446 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF841TWE 471 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF974AQC 517 bp overlap
ChIP dorsolateral prefrontal cortex ENCFF974AQC 390 bp overlap
ChIP gastroesophageal-sphincter ENCSR298ZPF.CTCF.gastroesophageal-sphincter 314 bp overlap
ChIP heart left ventricle ENCFF575JEQ 461 bp overlap
ChIP heart_left-ventricle ENCSR544APK.CTCF.heart_left-ventricle 279 bp overlap
ChIP heart_left-ventricle ENCSR791AYW.CTCF.heart_left-ventricle 182 bp overlap
ChIP heart_left-ventricle ENCSR718SDR.CTCF.heart_left-ventricle 202 bp overlap
ChIP hiPSC_IB12 GSE106870.CTCF.hiPSC_IB12 123 bp overlap
ChIP keratinocyte ENCSR000ALJ.CTCF.keratinocyte 177 bp overlap
ChIP pancreas_body ENCSR408XTO.CTCF.pancreas_body 148 bp overlap
ChIP testis ENCSR981CID.CTCF.testis 183 bp overlap
Cebpa 1 dataset
ChIP BLaER1 ENCFF031ISE 290 bp overlap
E2F1 1 dataset
ChIP U-87MG_GBM GSE99171.E2F1.U-87MG_GBM 232 bp overlap
EED 2 datasets
ChIP HepG2 ENCFF347CCA 545 bp overlap
ChIP HepG2 ENCFF347CCA 545 bp overlap
EGR3 1 dataset
Motif DE_48h DE_48h-EGR3_MA0732.2 11 bp overlap
EP300 1 dataset
ChIP sigmoid colon ENCFF524QSR 271 bp overlap
ESR1 1 dataset
ChIP Ishikawa_siETV4-72h GSE129803.ESR1.Ishikawa_siETV4-72h 226 bp overlap
EZH2 4 datasets
ChIP HepG2 ENCFF912EIW 166 bp overlap
ChIP endothelial_umbilical-vein ENCSR000ATA.EZH2.endothelial_umbilical-vein 184 bp overlap
ChIP keratinocyte ENCFF070STK 458 bp overlap
ChIP keratinocyte ENCFF070STK 570 bp overlap
FERD3L 1 dataset
Motif DE_48h DE_48h-FERD3L_MA1485.1 14 bp overlap
FOXP1 1 dataset
ChIP HepG2 ENCFF717IHQ 150 bp overlap
Foxn1 5 datasets
Motif DE_12h DE_12h-Foxn1_MA1684.1 6 bp overlap
Motif DE_36h DE_36h-Foxn1_MA1684.1 6 bp overlap
Motif DE_48h DE_48h-Foxn1_MA1684.1 6 bp overlap
Motif DE_60h DE_60h-Foxn1_MA1684.1 6 bp overlap
Motif DE_72h DE_72h-Foxn1_MA1684.1 6 bp overlap
GABPB1 1 dataset
ChIP HepG2 ENCFF315AWN 581 bp overlap
GRHL1 2 datasets
Motif DE_36h DE_36h-GRHL1_MA0647.2 10 bp overlap
Motif DE_60h DE_60h-GRHL1_MA0647.2 10 bp overlap
HOXA3 1 dataset
ChIP Hep-G2 ENCSR999JKC.HOXA3.Hep-G2 137 bp overlap
HSF1 1 dataset
ChIP NCI-H838 GSE38901.HSF1.NCI-H838 185 bp overlap
Hic1 5 datasets
Motif DE_12h DE_12h-Hic1_MA0739.2 8 bp overlap
Motif DE_36h DE_36h-Hic1_MA0739.2 8 bp overlap
Motif DE_48h DE_48h-Hic1_MA0739.2 8 bp overlap
Motif DE_60h DE_60h-Hic1_MA0739.2 8 bp overlap
Motif DE_72h DE_72h-Hic1_MA0739.2 8 bp overlap
JUN 2 datasets
ChIP ESC S34-ESC-d0-JUN-exp2 459 bp overlap
ChIP MDA-MB-231 GSE112444.JUN.MDA-MB-231 194 bp overlap
KDM4A 3 datasets
ChIP hiPSC_IA12 GSE106870.KDM4A.hiPSC_IA12 170 bp overlap
ChIP hiPSC_IIA11 GSE106870.KDM4A.hiPSC_IIA11 377 bp overlap
ChIP hiPSC_IID12 GSE106870.KDM4A.hiPSC_IID12 203 bp overlap
KDM5B 1 dataset
ChIP SUM185_SHCTCF GSE46055.KDM5B.SUM185_SHCTCF 141 bp overlap
KMT2A 1 dataset
ChIP CCRF-CEM GSE83671.KMT2A.CCRF-CEM 173 bp overlap
LIN54 5 datasets
Motif DE_12h DE_12h-LIN54_MA0619.2 7 bp overlap
Motif DE_36h DE_36h-LIN54_MA0619.2 7 bp overlap
Motif DE_48h DE_48h-LIN54_MA0619.2 7 bp overlap
Motif DE_60h DE_60h-LIN54_MA0619.2 7 bp overlap
Motif DE_72h DE_72h-LIN54_MA0619.2 7 bp overlap
MAX 5 datasets
ChIP HepG2 ENCFF479OHI 268 bp overlap
ChIP HepG2 ENCFF479OHI 485 bp overlap
ChIP WTC11 ENCFF223QFY 585 bp overlap
ChIP WTC11 ENCFF223QFY 403 bp overlap
ChIP WTC11 ENCFF223QFY 183 bp overlap
MEF2A 5 datasets
Motif DE_12h DE_12h-MEF2A_MA0052.5 10 bp overlap
Motif DE_36h DE_36h-MEF2A_MA0052.5 10 bp overlap
Motif DE_48h DE_48h-MEF2A_MA0052.5 10 bp overlap
Motif DE_60h DE_60h-MEF2A_MA0052.5 10 bp overlap
Motif DE_72h DE_72h-MEF2A_MA0052.5 10 bp overlap
MGA 2 datasets
ChIP HepG2 ENCFF057YJE 442 bp overlap
ChIP HepG2 ENCFF057YJE 642 bp overlap
MNX1 1 dataset
ChIP Hep-G2 ENCSR108MKR.MNX1.Hep-G2 250 bp overlap
MXD1 1 dataset
ChIP HepG2 ENCFF717MYN 545 bp overlap
MYC 1 dataset
ChIP CD34 GSE85488.MYC.CD34 178 bp overlap
MYCN 1 dataset
ChIP LA-N-5 GSE138295.MYCN.LA-N-5 345 bp overlap
MYOG 1 dataset
Motif DE_48h DE_48h-MYOG_MA0500.3 8 bp overlap
NFIA 2 datasets
Motif DE_36h DE_36h-NFIA_MA0670.2 6 bp overlap
Motif DE_60h DE_60h-NFIA_MA0670.2 6 bp overlap
NFIC 2 datasets
Motif DE_36h DE_36h-NFIC_MA0161.3 7 bp overlap
Motif DE_60h DE_60h-NFIC_MA0161.3 7 bp overlap
NFIX 2 datasets
Motif DE_36h DE_36h-NFIX_MA0671.2 6 bp overlap
Motif DE_60h DE_60h-NFIX_MA0671.2 6 bp overlap
NFYB 6 datasets
Motif DE_12h DE_12h-NFYB_MA0502.3 9 bp overlap
Motif DE_24h DE_24h-NFYB_MA0502.3 9 bp overlap
Motif DE_36h DE_36h-NFYB_MA0502.3 9 bp overlap
Motif DE_48h DE_48h-NFYB_MA0502.3 9 bp overlap
Motif DE_60h DE_60h-NFYB_MA0502.3 9 bp overlap
Motif DE_72h DE_72h-NFYB_MA0502.3 9 bp overlap
NHLH1 1 dataset
Motif DE_48h DE_48h-NHLH1_MA0048.3 9 bp overlap
NHLH2 1 dataset
Motif DE_48h DE_48h-NHLH2_MA1529.2 16 bp overlap
NR3C1 2 datasets
ChIP A-549 ENCSR000BJR.NR3C1.A-549 104 bp overlap
ChIP U2OS_SHHIC5 GSE65847.NR3C1.U2OS_SHHIC5 278 bp overlap
NRF1 21 datasets
ChIP GM12878 ENCFF969FRH 245 bp overlap
ChIP H1 ENCFF582PEJ 245 bp overlap
ChIP HMEC-1 GSE67867.NRF1.HMEC-1 385 bp overlap
ChIP HeLa_dC9Sun-D3A_TMEM206 GSE107607.NRF1.HeLa_dC9Sun-D3A_TMEM206 229 bp overlap
ChIP HeLa_dC9Sun-mCherry_TMEM206 GSE107607.NRF1.HeLa_dC9Sun-mCherry_TMEM206 206 bp overlap
ChIP Hep-G2 ENCSR853ADA.NRF1.Hep-G2 612 bp overlap
ChIP Hep-G2 GSE97661.NRF1.Hep-G2 612 bp overlap
ChIP HepG2 ENCFF694NVY 635 bp overlap
ChIP HepG2 ENCFF942ICJ 361 bp overlap
ChIP K-562 ENCSR837EYC.NRF1.K-562 530 bp overlap
ChIP K-562 ENCSR998AJK.NRF1.K-562 369 bp overlap
ChIP K-562 ENCSR494TDU.NRF1.K-562 349 bp overlap
ChIP K-562_Ab_R157-1-3D4 GSE97661.NRF1.K-562_Ab_R157-1-3D4 491 bp overlap
ChIP K-562_Ab_R157-1-3H1 GSE97661.NRF1.K-562_Ab_R157-1-3H1 118 bp overlap
ChIP K-562_Ab_R157-1-3H3 GSE97661.NRF1.K-562_Ab_R157-1-3H3 421 bp overlap
ChIP K562 ENCFF130SGK 409 bp overlap
ChIP K562 ENCFF689EWI 707 bp overlap
ChIP K562 ENCFF791UHF 680 bp overlap
ChIP MCF-7_Ab_R157-1-3D4 GSE97661.NRF1.MCF-7_Ab_R157-1-3D4 397 bp overlap
ChIP SK-N-SH ENCFF820YTU 125 bp overlap
ChIP SK-N-SH ENCSR000EHZ.NRF1.SK-N-SH 194 bp overlap
Nrf1 6 datasets
Motif DE_12h DE_12h-Nrf1_MA0506.3 12 bp overlap
Motif DE_24h DE_24h-Nrf1_MA0506.3 12 bp overlap
Motif DE_36h DE_36h-Nrf1_MA0506.3 12 bp overlap
Motif DE_48h DE_48h-Nrf1_MA0506.3 12 bp overlap
Motif DE_60h DE_60h-Nrf1_MA0506.3 12 bp overlap
Motif DE_72h DE_72h-Nrf1_MA0506.3 12 bp overlap
OLIG2 2 datasets
ChIP brain-prefrontal-cortex_2017011 GSE129039.OLIG2.brain-prefrontal-cortex_2017011 260 bp overlap
ChIP brain-prefrontal-cortex_2017014 GSE129039.OLIG2.brain-prefrontal-cortex_2017014 297 bp overlap
Olig2 1 dataset
Motif DE_48h DE_48h-Olig2_MA1997.2 6 bp overlap
PGR 1 dataset
ChIP myometrium_NP2 GSE137550.PGR.myometrium_NP2 272 bp overlap
POLR2A 6 datasets
ChIP esophagus muscularis mucosa ENCFF759BBR 188 bp overlap
ChIP esophagus muscularis mucosa ENCFF759BBR 391 bp overlap
ChIP prostate gland ENCFF881OMH 417 bp overlap
ChIP sigmoid colon ENCFF653CQA 425 bp overlap
ChIP sigmoid colon ENCFF754JQR 365 bp overlap
ChIP spleen ENCFF446ZGT 284 bp overlap
POU5F1 1 dataset
ChIP BG03 GSE21614.POU5F1.BG03 153 bp overlap
POU5F1_M 1 dataset
ChIP DE_D1 DED1-OCT4-M_Batch_II 546 bp overlap
PTBP1 1 dataset
ChIP Hep-G2 ENCSR156APP.PTBP1.Hep-G2 170 bp overlap
RAD21 1 dataset
ChIP hiPSC_IIIB12 GSE106870.RAD21.hiPSC_IIIB12 146 bp overlap
RBM39 2 datasets
ChIP Hep-G2 GSE120104.RBM39.Hep-G2 220 bp overlap
ChIP Hep-G2 ENCSR339JTP.RBM39.Hep-G2 233 bp overlap
RBPJ 8 datasets
Motif DE_12h DE_12h-RBPJ_MA1116.2 6 bp overlap
Motif DE_36h DE_36h-RBPJ_MA1116.2 6 bp overlap
Motif DE_48h DE_48h-RBPJ_MA1116.2 6 bp overlap
Motif DE_60h DE_60h-RBPJ_MA1116.2 6 bp overlap
Motif DE_72h DE_72h-RBPJ_MA1116.2 6 bp overlap
ChIP GSC8-11_12d-das GSE74557.RBPJ.GSC8-11_12d-das 309 bp overlap
ChIP GSC8-11_dasatinib GSE74557.RBPJ.GSC8-11_dasatinib 223 bp overlap
ChIP NHEK GSE29498.RBPJ.NHEK 110 bp overlap
RELA 4 datasets
ChIP KB GSE52469.RELA.KB 129 bp overlap
ChIP KB_5Z GSE64223.RELA.KB_5Z 113 bp overlap
ChIP KB_5Z_IL GSE64223.RELA.KB_5Z_IL 140 bp overlap
ChIP KB_IL GSE52469.RELA.KB_IL 153 bp overlap
RFX1 3 datasets
Motif DE_36h DE_36h-RFX1_MA0509.3 16 bp overlap
Motif DE_60h DE_60h-RFX1_MA0509.3 16 bp overlap
ChIP K562 ENCFF421AVO 465 bp overlap
RFX2 2 datasets
Motif DE_36h DE_36h-RFX2_MA0600.3 14 bp overlap
Motif DE_60h DE_60h-RFX2_MA0600.3 14 bp overlap
RFX3 2 datasets
Motif DE_36h DE_36h-RFX3_MA0798.3 16 bp overlap
Motif DE_60h DE_60h-RFX3_MA0798.3 16 bp overlap
RFX5 2 datasets
Motif DE_36h DE_36h-RFX5_MA0510.3 14 bp overlap
Motif DE_60h DE_60h-RFX5_MA0510.3 14 bp overlap
SMAD3 1 dataset
ChIP H69_5TGF GSE145250.SMAD3.H69_5TGF 180 bp overlap
SPIC 5 datasets
Motif DE_12h DE_12h-SPIC_MA0687.2 13 bp overlap
Motif DE_36h DE_36h-SPIC_MA0687.2 13 bp overlap
Motif DE_48h DE_48h-SPIC_MA0687.2 13 bp overlap
Motif DE_60h DE_60h-SPIC_MA0687.2 13 bp overlap
Motif DE_72h DE_72h-SPIC_MA0687.2 13 bp overlap
STAG2 4 datasets
ChIP HCAEC GSE101921.STAG2.HCAEC 341 bp overlap
ChIP MCF-10A_Control GSE101921.STAG2.MCF-10A_Control 213 bp overlap
ChIP MCF-10A_siSTAG1 GSE101921.STAG2.MCF-10A_siSTAG1 192 bp overlap
ChIP MCF-10A_siSTAG2 GSE101921.STAG2.MCF-10A_siSTAG2 193 bp overlap
STAT3 1 dataset
ChIP HCC1187 GSE152203.STAT3.HCC1187 223 bp overlap
TAF15 2 datasets
ChIP Hep-G2 GSE120104.TAF15.Hep-G2 198 bp overlap
ChIP Hep-G2 ENCSR825MZS.TAF15.Hep-G2 190 bp overlap
TFAP2C 1 dataset
ChIP UCLA1-hESCs_TFAP2C--_1d_TFAP2C_Induc GSE101074.TFAP2C.UCLA1-hESCs_TFAP2C--_1d_TFAP2C_Induc 235 bp overlap
Tcf12 1 dataset
Motif DE_48h DE_48h-Tcf12_MA0521.3 6 bp overlap
Twist2 1 dataset
Motif DE_48h DE_48h-Twist2_MA0633.3 6 bp overlap
USF2 1 dataset
ChIP IMR-90 ENCSR513UQG.USF2.IMR-90 113 bp overlap
ZBTB48 1 dataset
ChIP U2OS GSE96776.ZBTB48.U2OS 376 bp overlap
ZFP91 3 datasets
ChIP HepG2 ENCFF012CME 497 bp overlap
ChIP K-562 ENCSR898XMH.ZFP91.K-562 302 bp overlap
ChIP K562 ENCFF501CDP 214 bp overlap
ZFY 1 dataset
ChIP Hep-G2 ENCSR949OEV.ZFY.Hep-G2 159 bp overlap
ZNF225 1 dataset
ChIP HepG2 ENCFF500HTT 309 bp overlap
ZNF320 1 dataset
Motif DE_48h DE_48h-ZNF320_MA1976.2 20 bp overlap
ZNF639 2 datasets
ChIP K-562 ENCSR949NVY.ZNF639.K-562 347 bp overlap
ChIP K562 ENCFF267NLX 314 bp overlap
ZNF687 1 dataset
ChIP HepG2 ENCFF653WIX 728 bp overlap
ZNF707 6 datasets
Motif DE_12h DE_12h-ZNF707_MA1715.1 15 bp overlap
Motif DE_24h DE_24h-ZNF707_MA1715.1 15 bp overlap
Motif DE_36h DE_36h-ZNF707_MA1715.1 15 bp overlap
Motif DE_48h DE_48h-ZNF707_MA1715.1 15 bp overlap
Motif DE_60h DE_60h-ZNF707_MA1715.1 15 bp overlap
Motif DE_72h DE_72h-ZNF707_MA1715.1 15 bp overlap
ZNF708 6 datasets
Motif DE_12h DE_12h-ZNF708_MA1730.2 9 bp overlap
Motif DE_24h DE_24h-ZNF708_MA1730.2 9 bp overlap
Motif DE_36h DE_36h-ZNF708_MA1730.2 9 bp overlap
Motif DE_48h DE_48h-ZNF708_MA1730.2 9 bp overlap
Motif DE_60h DE_60h-ZNF708_MA1730.2 9 bp overlap
Motif DE_72h DE_72h-ZNF708_MA1730.2 9 bp overlap
ZNF93 1 dataset
Motif DE_48h DE_48h-ZNF93_MA1721.2 14 bp overlap
Zfx 6 datasets
Motif DE_12h DE_12h-Zfx_MA0146.3 10 bp overlap
Motif DE_24h DE_24h-Zfx_MA0146.3 10 bp overlap
Motif DE_36h DE_36h-Zfx_MA0146.3 10 bp overlap
Motif DE_48h DE_48h-Zfx_MA0146.3 10 bp overlap
Motif DE_60h DE_60h-Zfx_MA0146.3 10 bp overlap
Motif DE_72h DE_72h-Zfx_MA0146.3 10 bp overlap
Zic1::Zic2 3 datasets
Motif DE_36h DE_36h-Zic1Zic2_MA1628.2 7 bp overlap
Motif DE_48h DE_48h-Zic1Zic2_MA1628.2 7 bp overlap
Motif DE_60h DE_60h-Zic1Zic2_MA1628.2 7 bp overlap
Zic3 3 datasets
Motif DE_36h DE_36h-Zic3_MA0697.3 7 bp overlap
Motif DE_48h DE_48h-Zic3_MA0697.3 7 bp overlap
Motif DE_60h DE_60h-Zic3_MA0697.3 7 bp overlap