This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for EP300. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EP300 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EP300, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:40,818,885–40,819,668 | 273.1 kb | Distal (>10kb) Multiome | 683 | |
| chr22:40,856,149–40,857,393 | 235.7 kb | Distal (>10kb) Multiome | 950 | |
| chr22:40,950,581–40,952,237 | 141.1 kb | Distal (>10kb) Multiome | 884 | |
| chr22:41,021,558–41,023,199 | 70.5 kb | Distal (>10kb) Multiome | 978 | |
| chr22:41,091,127–41,092,906 | 901 bp | At TSS Multiome | 880 | |
| chr22:41,197,028–41,198,019 | 105.0 kb | Distal (>10kb) Multiome | 640 | |
| chr22:41,204,902–41,205,619 | 112.7 kb | Distal (>10kb) Multiome | 640 | |
| chr22:41,237,372–41,238,796 | 145.3 kb | Distal (>10kb) Multiome | 302 | |
| chr22:41,285,539–41,286,833 | 193.8 kb | Distal (>10kb) Multiome | 879 | |
| chr22:41,301,155–41,301,870 | 209.0 kb | Distal (>10kb) Multiome | 658 | |
| chr22:41,380,458–41,382,737 | 289.2 kb | Distal (>10kb) Multiome | 925 |
Genomic view of the EP300 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.