The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NFYB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFYB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFYB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:103,840,241–103,842,325 | 296.9 kb | Distal (>10kb) Multiome | 911 | |
| chr12:103,928,943–103,931,279 | 208.0 kb | Distal (>10kb) Multiome | 1072 | |
| chr12:103,956,327–103,957,900 | 180.9 kb | Distal (>10kb) Multiome | 1090 | |
| chr12:103,965,409–103,966,472 | 172.3 kb | Distal (>10kb) Multiome | 965 | |
| chr12:104,049,717–104,050,283 | 88.2 kb | Distal (>10kb) Multiome | 295 | |
| chr12:104,063,714–104,065,209 | 73.7 kb | Distal (>10kb) Multiome | 950 | |
| chr12:104,137,119–104,139,117 | 9 bp | At TSS Multiome | 796 | |
| chr12:104,154,657–104,155,425 | 16.8 kb | Distal (>10kb) Multiome | 127 | |
| chr12:104,215,473–104,216,381 | 77.6 kb | Distal (>10kb) Multiome | 514 | |
| chr12:104,286,560–104,287,505 | 148.7 kb | Distal (>10kb) Multiome | 878 | |
| chr12:104,288,643–104,289,175 | 150.6 kb | Distal (>10kb) Multiome | 863 | |
| chr12:104,303,321–104,304,194 | 165.5 kb | Distal (>10kb) Multiome | 316 | |
| chr12:104,408,645–104,409,206 | 270.6 kb | Distal (>10kb) Multiome | 348 | |
| chr12:104,414,328–104,416,462 | 276.6 kb | Distal (>10kb) Multiome | 437 | |
| chr12:104,957,697–104,958,992 | 820.1 kb | Distal (>10kb) Multiome HiCAR | 811 | |
| chr12:105,107,164–105,108,312 | 969.5 kb | Distal (>10kb) Multiome HiCAR | 1033 |
Genomic view of the NFYB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.