E2F1

E2F transcription factor 1 | RBP3, RBBP3

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Member of: DE-6 DE-6.14

Biological processes 68 terms

DNA binding (GO:0003677)DNA binding (GO:0003677)DNA damage checkpoint signaling (GO:0000077)DNA-binding transcription activator activity (GO:0001216)DNA-binding transcription activator activity (GO:0001216)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor binding (GO:0140297)DNA-templated transcription (GO:0006351)DNA-templated transcription (GO:0006351)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)Rb-E2F complex (GO:0035189)Rb-E2F complex (GO:0035189)cellular response to fatty acid (GO:0071398)cellular response to hypoxia (GO:0071456)cellular response to nerve growth factor stimulus (GO:1990090)chromatin (GO:0000785)chromatin (GO:0000785)chromatin (GO:0000785)cis-regulatory region sequence-specific DNA binding (GO:0000987)cytoplasm (GO:0005737)intrinsic apoptotic signaling pathway in response to DNA damage (GO:0008630)mRNA stabilization (GO:0048255)molecular adaptor activity (GO:0060090)negative regulation of DNA binding (GO:0043392)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of fat cell differentiation (GO:0045599)negative regulation of fat cell differentiation (GO:0045599)negative regulation of fat cell proliferation (GO:0070345)negative regulation of fat cell proliferation (GO:0070345)negative regulation of transcription by RNA polymerase II (GO:0000122)nuclear chromosome (GO:0000228)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of apoptotic process (GO:0043065)positive regulation of fibroblast proliferation (GO:0048146)positive regulation of gene expression (GO:0010628)positive regulation of glial cell proliferation (GO:0060252)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein dimerization activity (GO:0046983)protein kinase binding (GO:0019901)protein-containing complex (GO:0032991)regulation of DNA-templated transcription (GO:0006355)regulation of DNA-templated transcription (GO:0006355)regulation of G1/S transition of mitotic cell cycle (GO:2000045)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)response to lipopolysaccharide (GO:0032496)sequence-specific DNA binding (GO:0043565)sequence-specific DNA binding (GO:0043565)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)spermatogenesis (GO:0007283)transcription cis-regulatory region binding (GO:0000976)transcription regulator complex (GO:0005667)

Expression (TPM)

TFs regulating E2F1 0 TFs

Transcription factors with Perturb-seq knockdown data for E2F1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = E2F1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to E2F1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of E2F1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr20:33,443,149–33,444,777	242.5 kb	Distal (>10kb) Multiome	726
chr20:33,475,400–33,476,091	210.6 kb	Distal (>10kb) Multiome	98
chr20:33,477,323–33,477,813	208.8 kb	Distal (>10kb) Multiome	124
chr20:33,489,654–33,490,762	196.4 kb	Distal (>10kb) Multiome	1000
chr20:33,663,243–33,664,235	22.7 kb	Distal (>10kb) Multiome	895
chr20:33,666,358–33,668,405	19.6 kb	Distal (>10kb) Multiome	603
chr20:33,673,732–33,675,136	11.9 kb	Distal (>10kb) Multiome	826
chr20:33,685,755–33,686,963	158 bp	At TSS Multiome	830
chr20:33,720,042–33,720,684	33.9 kb	Distal (>10kb) Multiome	584
chr20:33,731,496–33,732,906	45.8 kb	Distal (>10kb) Multiome	1049
chr20:33,811,016–33,811,951	124.9 kb	Distal (>10kb) Multiome	921

Genome Browser

Genomic view of the E2F1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.