Transcription factors with Perturb-seq knockdown data for C4orf50. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C4orf50 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C4orf50, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:5,943,556–5,944,317 | 256.7 kb | Distal (>10kb) Multiome | 79 | |
| chr4:6,116,627–6,117,904 | 83.2 kb | Distal (>10kb) Multiome | 223 | |
| chr4:6,136,985–6,138,128 | 63.0 kb | Distal (>10kb) Multiome | 150 | |
| chr4:6,182,458–6,183,222 | 17.8 kb | Distal (>10kb) Multiome | 84 | |
| chr4:6,197,469–6,197,705 | 2.8 kb | Proximal (<10kb) | 12 | |
| chr4:6,198,414–6,201,258 | 89 bp | At TSS Multiome | 514 | |
| chr4:6,201,655–6,202,156 | 1.1 kb | Proximal (<10kb) | 72 | |
| chr4:6,203,985–6,211,095 | 3.8 kb | Proximal (<10kb) Multiome | 148 | |
| chr4:6,221,494–6,222,648 | 21.4 kb | Distal (>10kb) Multiome | 327 | |
| chr4:6,245,263–6,246,104 | 45.2 kb | Distal (>10kb) Multiome | 152 | |
| chr4:6,269,131–6,270,539 | 69.2 kb | Distal (>10kb) Multiome | 561 | |
| chr4:6,308,663–6,309,660 | 108.6 kb | Distal (>10kb) Multiome | 421 | |
| chr4:6,327,148–6,327,740 | 126.9 kb | Distal (>10kb) Multiome | 371 | |
| chr4:6,367,437–6,367,936 | 167.1 kb | Distal (>10kb) Multiome | 38 | |
| chr4:6,470,551–6,471,324 | 270.4 kb | Distal (>10kb) Multiome | 373 | |
| chr4:6,472,122–6,472,949 | 272.0 kb | Distal (>10kb) Multiome | 157 |
Genomic view of the C4orf50 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.