This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]
Transcription factors with Perturb-seq knockdown data for AMFR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AMFR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AMFR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:56,190,607–56,192,076 | 234.2 kb | Distal (>10kb) Multiome | 450 | |
| chr16:56,194,047–56,194,919 | 231.0 kb | Distal (>10kb) Multiome | 346 | |
| chr16:56,281,237–56,281,831 | 143.9 kb | Distal (>10kb) Multiome | 230 | |
| chr16:56,289,711–56,290,418 | 135.5 kb | Distal (>10kb) Multiome | 224 | |
| chr16:56,318,032–56,318,622 | 107.1 kb | Distal (>10kb) Multiome | 401 | |
| chr16:56,356,409–56,357,020 | 68.9 kb | Distal (>10kb) Multiome | 736 | |
| chr16:56,422,952–56,423,244 | 2.3 kb | Proximal (<10kb) | 56 | |
| chr16:56,424,368–56,425,945 | 42 bp | At TSS Multiome | 582 | |
| chr16:56,450,882–56,452,062 | 25.9 kb | Distal (>10kb) Multiome | 828 | |
| chr16:56,519,537–56,520,432 | 94.4 kb | Distal (>10kb) Multiome | 798 | |
| chr16:56,547,229–56,547,931 | 122.1 kb | Distal (>10kb) Multiome | 198 | |
| chr16:56,608,175–56,608,748 | 183.0 kb | Distal (>10kb) Multiome | 861 | |
| chr16:56,625,188–56,625,919 | 200.1 kb | Distal (>10kb) Multiome | 474 | |
| chr16:56,632,101–56,632,676 | 206.9 kb | Distal (>10kb) Multiome | 180 | |
| chr16:56,657,372–56,658,310 | 232.2 kb | Distal (>10kb) Multiome | 494 | |
| chr16:56,668,023–56,668,619 | 242.6 kb | Distal (>10kb) Multiome | 379 | |
| chr16:56,675,665–56,676,180 | 250.3 kb | Distal (>10kb) Multiome | 97 | |
| chr16:56,681,954–56,682,716 | 256.8 kb | Distal (>10kb) Multiome | 699 |
Genomic view of the AMFR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.