The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome. [provided by RefSeq, Dec 2016]
Transcription factors with Perturb-seq knockdown data for USP44. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = USP44 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of USP44, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:95,442,539–95,444,040 | 105.2 kb | Distal (>10kb) Multiome | 107 | |
| chr12:95,446,296–95,447,252 | 102.1 kb | Distal (>10kb) Multiome | 289 | |
| chr12:95,473,495–95,474,647 | 74.8 kb | Distal (>10kb) Multiome | 953 | |
| chr12:95,538,674–95,538,950 | 9.9 kb | Proximal (<10kb) | 32 | |
| chr12:95,547,928–95,549,389 | 75 bp | At TSS Multiome | 471 | |
| chr12:95,549,780–95,549,971 | 961 bp | At TSS | 115 | |
| chr12:95,551,266–95,551,780 | 2.7 kb | Proximal (<10kb) Multiome | 171 | |
| chr12:95,745,876–95,746,868 | 197.6 kb | Distal (>10kb) Multiome | 68 | |
| chr12:95,789,276–95,791,638 | 241.7 kb | Distal (>10kb) Multiome | 591 |
Genomic view of the USP44 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.