This gene encodes a cytoplasmic protein implicated in regulation of the mitotic spindle checkpoint, a regulatory pathway that monitors chromosome segregation during cell division to ensure the proper distribution of chromosomes to daughter cells. The protein is phosphorylated in mitosis and in response to activation of the spindle checkpoint, and disappears when cells transition to G1 phase. It interacts with a mitotic regulator, and its expression is required to efficiently activate the spindle checkpoint. It is proposed to regulate Cdc2 kinase activity during spindle checkpoint arrest. Chromosomal deletions that fuse this gene and the adjacent locus commonly occur in T cell leukemias, and are thought to arise through illegitimate V-(D)-J recombination events. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for STIL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = STIL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of STIL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:47,023,511–47,024,146 | 290.4 kb | Distal (>10kb) Multiome | 258 | |
| chr1:47,179,069–47,179,667 | 134.8 kb | Distal (>10kb) Multiome | 660 | |
| chr1:47,192,732–47,193,390 | 121.1 kb | Distal (>10kb) Multiome | 573 | |
| chr1:47,208,388–47,208,956 | 105.5 kb | Distal (>10kb) Multiome | 76 | |
| chr1:47,307,736–47,308,358 | 6.1 kb | Proximal (<10kb) Multiome | 63 | |
| chr1:47,313,466–47,313,686 | 460 bp | At TSS | 285 | |
| chr1:47,313,819–47,314,595 | 140 bp | At TSS Multiome | 830 | |
| chr1:47,314,722–47,315,042 | 575 bp | At TSS | 258 | |
| chr1:47,333,339–47,334,866 | 19.8 kb | Distal (>10kb) Multiome | 912 | |
| chr1:47,416,109–47,417,449 | 102.7 kb | Distal (>10kb) Multiome | 138 | |
| chr1:47,424,446–47,424,949 | 110.5 kb | Distal (>10kb) Multiome | 274 | |
| chr1:47,432,258–47,433,058 | 118.5 kb | Distal (>10kb) Multiome | 298 | |
| chr1:47,436,505–47,439,686 | 123.7 kb | Distal (>10kb) Multiome | 844 | |
| chr1:47,449,968–47,450,449 | 136.0 kb | Distal (>10kb) Multiome | 195 | |
| chr1:47,523,548–47,524,329 | 209.9 kb | Distal (>10kb) Multiome | 53 | |
| chr1:47,532,955–47,534,409 | 219.4 kb | Distal (>10kb) Multiome | 323 | |
| chr1:47,593,089–47,593,546 | 279.1 kb | Distal (>10kb) Multiome | 136 |
Genomic view of the STIL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.