SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for SPRED2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPRED2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPRED2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:65,227,140–65,228,436 | 204.9 kb | Distal (>10kb) Multiome | 971 | |
| chr2:65,331,150–65,331,849 | 101.1 kb | Distal (>10kb) Multiome | 47 | |
| chr2:65,350,820–65,351,607 | 81.3 kb | Distal (>10kb) Multiome HiCAR | 223 | |
| chr2:65,430,513–65,433,282 | 95 bp | At TSS Multiome | 1068 | |
| chr2:65,434,642–65,434,812 | 2.0 kb | Proximal (<10kb) | 34 | |
| chr2:65,435,385–65,436,948 | 3.4 kb | Proximal (<10kb) Multiome | 1075 | |
| chr2:65,437,559–65,438,643 | 5.4 kb | Proximal (<10kb) Multiome | 402 | |
| chr2:65,558,091–65,558,575 | 125.7 kb | Distal (>10kb) Multiome | 55 | |
| chr2:65,566,479–65,567,273 | 134.2 kb | Distal (>10kb) Multiome HiCAR | 185 | |
| chr2:65,579,271–65,580,350 | 147.2 kb | Distal (>10kb) Multiome | 271 |
Genomic view of the SPRED2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.