RSPH3
radial spoke head 3 | RSP3, dJ111C20.1, RSHL2

The protein encoded by this gene acts as a protein kinase A anchoring protein. Mutations in this gene cause primary ciliary dyskinesia; a disorder characterized by defects of the axoneme in motile cilia and sperm flagella. The homolog of this gene was first identified in the blue-green algae Chlamydomonas as encoding a radial spoke protein that formed a structural component of motile cilia and flagella. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Dec 2016]

Member of: DE-10 DE-10.5 Developmental clusters: GC6
Biological processes 12 terms
Expression (TPM)
RSPH3 — as a Regulated Gene

TFs regulating RSPH3 0 TFs

Transcription factors with Perturb-seq knockdown data for RSPH3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RSPH3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to RSPH3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RSPH3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr6:158,704,230–158,704,856 295.8 kb Distal (>10kb) Multiome HiCAR 886
chr6:158,706,215–158,706,876 293.8 kb Distal (>10kb) Multiome HiCAR 190
chr6:158,707,164–158,707,736 292.7 kb Distal (>10kb) Multiome HiCAR 295
chr6:158,718,163–158,718,988 281.6 kb Distal (>10kb) Multiome HiCAR 244
chr6:158,773,639–158,774,564 226.0 kb Distal (>10kb) Multiome HiCAR 55
chr6:158,817,287–158,819,780 181.9 kb Distal (>10kb) Multiome 1077
chr6:158,835,350–158,835,822 164.5 kb Distal (>10kb) Multiome 212
chr6:158,853,179–158,854,186 146.7 kb Distal (>10kb) Multiome 879
chr6:158,869,125–158,870,412 130.4 kb Distal (>10kb) Multiome 808
chr6:158,878,392–158,879,559 121.4 kb Distal (>10kb) Multiome 84
chr6:158,910,515–158,910,986 89.5 kb Distal (>10kb) Multiome 244
chr6:158,994,524–158,994,660 5.1 kb Proximal (<10kb) 5
chr6:158,994,914–158,995,128 4.7 kb Proximal (<10kb) 11
chr6:158,999,160–159,000,539 274 bp At TSS Multiome 1122
chr6:159,103,945–159,104,600 104.1 kb Distal (>10kb) Multiome HiCAR 318
chr6:159,168,575–159,170,383 169.2 kb Distal (>10kb) Multiome 353
chr6:159,233,512–159,234,391 233.7 kb Distal (>10kb) Multiome 147

Genome Browser

Genomic view of the RSPH3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr6:158,694,230 – 159,244,391
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq