This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]
Transcription factors with Perturb-seq knockdown data for PIF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:64,588,906–64,589,753 | 236.3 kb | Distal (>10kb) Multiome | 112 | |
| chr15:64,634,613–64,635,221 | 190.8 kb | Distal (>10kb) Multiome | 309 | |
| chr15:64,699,707–64,700,384 | 125.5 kb | Distal (>10kb) Multiome | 205 | |
| chr15:64,702,680–64,704,073 | 122.3 kb | Distal (>10kb) Multiome | 747 | |
| chr15:64,774,780–64,776,025 | 50.0 kb | Distal (>10kb) Multiome | 323 | |
| chr15:64,776,175–64,776,789 | 49.0 kb | Distal (>10kb) Multiome | 105 | |
| chr15:64,780,613–64,781,099 | 44.8 kb | Distal (>10kb) Multiome | 74 | |
| chr15:64,807,123–64,808,403 | 17.7 kb | Distal (>10kb) Multiome | 248 | |
| chr15:64,808,791–64,809,297 | 16.5 kb | Distal (>10kb) Multiome | 89 | |
| chr15:64,809,806–64,810,371 | 15.5 kb | Distal (>10kb) Multiome | 298 | |
| chr15:64,823,825–64,824,470 | 1.5 kb | Proximal (<10kb) Multiome | 206 | |
| chr15:64,825,130–64,826,273 | 216 bp | At TSS Multiome | 784 | |
| chr15:64,840,757–64,843,201 | 16.0 kb | Distal (>10kb) Multiome | 732 | |
| chr15:64,893,790–64,894,635 | 68.6 kb | Distal (>10kb) Multiome | 506 | |
| chr15:64,905,276–64,905,958 | 80.0 kb | Distal (>10kb) Multiome | 362 | |
| chr15:64,911,481–64,912,188 | 86.2 kb | Distal (>10kb) Multiome | 308 | |
| chr15:64,915,576–64,916,281 | 90.3 kb | Distal (>10kb) Multiome | 115 | |
| chr15:64,989,345–64,990,371 | 164.3 kb | Distal (>10kb) Multiome | 859 | |
| chr15:65,028,915–65,029,974 | 203.9 kb | Distal (>10kb) Multiome | 703 | |
| chr15:65,049,359–65,049,794 | 223.9 kb | Distal (>10kb) Multiome | 267 | |
| chr15:65,067,081–65,068,384 | 242.5 kb | Distal (>10kb) Multiome | 131 | |
| chr15:65,101,638–65,102,425 | 276.3 kb | Distal (>10kb) Multiome | 204 |
Genomic view of the PIF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.