The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Transcription factors with Perturb-seq knockdown data for PFKFB4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PFKFB4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PFKFB4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:48,300,947–48,301,746 | 255.6 kb | Distal (>10kb) Multiome | 954 | |
| chr3:48,372,864–48,373,543 | 183.8 kb | Distal (>10kb) Multiome | 49 | |
| chr3:48,428,528–48,430,504 | 127.0 kb | Distal (>10kb) Multiome | 910 | |
| chr3:48,439,801–48,440,548 | 116.8 kb | Distal (>10kb) Multiome | 787 | |
| chr3:48,446,396–48,447,180 | 110.3 kb | Distal (>10kb) Multiome | 767 | |
| chr3:48,465,344–48,466,310 | 91.3 kb | Distal (>10kb) Multiome HiCAR | 670 | |
| chr3:48,472,636–48,473,658 | 83.8 kb | Distal (>10kb) Multiome HiCAR | 821 | |
| chr3:48,503,451–48,504,839 | 52.9 kb | Distal (>10kb) Multiome | 419 | |
| chr3:48,548,798–48,549,027 | 8.0 kb | Proximal (<10kb) | 219 | |
| chr3:48,556,022–48,557,666 | 28 bp | At TSS Multiome | 794 | |
| chr3:48,558,131–48,558,793 | 1.4 kb | Proximal (<10kb) Multiome | 503 | |
| chr3:48,561,048–48,561,323 | 4.0 kb | Proximal (<10kb) | 275 | |
| chr3:48,608,816–48,610,628 | 52.6 kb | Distal (>10kb) Multiome | 850 | |
| chr3:48,634,696–48,636,251 | 78.6 kb | Distal (>10kb) Multiome | 898 | |
| chr3:48,655,787–48,657,257 | 99.4 kb | Distal (>10kb) Multiome | 438 | |
| chr3:48,661,275–48,664,636 | 104.7 kb | Distal (>10kb) Multiome | 1248 | |
| chr3:48,685,362–48,686,531 | 128.9 kb | Distal (>10kb) Multiome | 770 | |
| chr3:48,716,958–48,717,863 | 160.3 kb | Distal (>10kb) Multiome | 634 | |
| chr3:48,847,059–48,848,224 | 290.8 kb | Distal (>10kb) Multiome | 772 |
Genomic view of the PFKFB4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.