Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
Transcription factors with Perturb-seq knockdown data for PDK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PDK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PDK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:172,380,587–172,381,629 | 175.0 kb | Distal (>10kb) Multiome | 280 | |
| chr2:172,427,119–172,428,808 | 128.4 kb | Distal (>10kb) Multiome | 799 | |
| chr2:172,429,611–172,430,353 | 126.0 kb | Distal (>10kb) Multiome | 101 | |
| chr2:172,434,900–172,435,930 | 120.5 kb | Distal (>10kb) Multiome | 213 | |
| chr2:172,549,606–172,550,190 | 5.8 kb | Proximal (<10kb) | 15 | |
| chr2:172,550,527–172,550,829 | 5.2 kb | Proximal (<10kb) | 29 | |
| chr2:172,554,417–172,555,143 | 1.3 kb | Proximal (<10kb) Multiome | 179 | |
| chr2:172,555,296–172,556,851 | 4 bp | At TSS Multiome | 1053 | |
| chr2:172,674,529–172,675,178 | 118.8 kb | Distal (>10kb) Multiome | 202 |
Genomic view of the PDK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.