This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for GJA4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GJA4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GJA4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:34,742,694–34,743,170 | 50.1 kb | Distal (>10kb) Multiome | 266 | |
| chr1:34,749,736–34,750,608 | 42.9 kb | Distal (>10kb) Multiome | 52 | |
| chr1:34,774,067–34,774,642 | 18.7 kb | Distal (>10kb) Multiome | 20 | |
| chr1:34,781,173–34,782,115 | 11.3 kb | Distal (>10kb) Multiome | 263 | |
| chr1:34,786,486–34,786,748 | 6.2 kb | Proximal (<10kb) | 257 | |
| chr1:34,792,757–34,793,260 | 68 bp | At TSS Multiome | 67 | |
| chr1:34,843,142–34,844,904 | 51.4 kb | Distal (>10kb) Multiome HiCAR | 414 | |
| chr1:34,846,880–34,847,412 | 54.1 kb | Distal (>10kb) Multiome HiCAR | 93 | |
| chr1:34,852,691–34,853,220 | 59.9 kb | Distal (>10kb) Multiome HiCAR | 557 | |
| chr1:34,859,367–34,859,973 | 66.8 kb | Distal (>10kb) Multiome HiCAR | 862 | |
| chr1:34,865,980–34,866,815 | 73.4 kb | Distal (>10kb) Multiome HiCAR | 588 | |
| chr1:34,885,018–34,886,281 | 92.8 kb | Distal (>10kb) Multiome | 254 | |
| chr1:34,929,050–34,930,777 | 136.6 kb | Distal (>10kb) Multiome | 401 | |
| chr1:34,984,883–34,985,660 | 192.3 kb | Distal (>10kb) Multiome | 682 | |
| chr1:35,031,470–35,032,157 | 238.8 kb | Distal (>10kb) Multiome | 762 | |
| chr1:35,079,136–35,079,827 | 286.4 kb | Distal (>10kb) Multiome | 764 | |
| chr1:35,556,848–35,558,023 | 764.6 kb | Distal (>10kb) Multiome HiCAR | 894 |
Genomic view of the GJA4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.