Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for CHAF1A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHAF1A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHAF1A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:4,123,681–4,124,581 | 278.5 kb | Distal (>10kb) Multiome | 798 | |
| chr19:4,182,102–4,182,890 | 220.1 kb | Distal (>10kb) Multiome | 862 | |
| chr19:4,198,066–4,198,600 | 204.3 kb | Distal (>10kb) Multiome | 214 | |
| chr19:4,246,623–4,247,617 | 155.4 kb | Distal (>10kb) Multiome | 960 | |
| chr19:4,326,625–4,329,107 | 74.2 kb | Distal (>10kb) Multiome | 567 | |
| chr19:4,342,323–4,344,016 | 59.7 kb | Distal (>10kb) Multiome | 969 | |
| chr19:4,390,525–4,391,068 | 12.0 kb | Distal (>10kb) Multiome | 464 | |
| chr19:4,399,835–4,401,019 | 2.0 kb | Proximal (<10kb) Multiome | 828 | |
| chr19:4,402,106–4,403,050 | 125 bp | At TSS Multiome | 731 | |
| chr19:4,456,541–4,458,172 | 55.1 kb | Distal (>10kb) Multiome | 757 | |
| chr19:4,471,202–4,472,545 | 69.3 kb | Distal (>10kb) Multiome | 715 | |
| chr19:4,474,252–4,474,874 | 72.1 kb | Distal (>10kb) Multiome | 201 | |
| chr19:4,534,817–4,535,416 | 132.6 kb | Distal (>10kb) Multiome | 451 | |
| chr19:4,556,056–4,556,977 | 154.0 kb | Distal (>10kb) Multiome | 259 | |
| chr19:4,581,475–4,581,916 | 179.1 kb | Distal (>10kb) Multiome | 358 | |
| chr19:4,597,380–4,598,540 | 195.5 kb | Distal (>10kb) Multiome | 280 | |
| chr19:4,633,303–4,633,826 | 230.8 kb | Distal (>10kb) Multiome | 500 | |
| chr19:4,636,101–4,636,717 | 233.8 kb | Distal (>10kb) Multiome | 445 | |
| chr19:4,639,001–4,640,103 | 236.7 kb | Distal (>10kb) Multiome | 710 | |
| chr19:4,669,686–4,671,221 | 267.8 kb | Distal (>10kb) Multiome | 797 | |
| chr19:4,678,652–4,679,681 | 276.5 kb | Distal (>10kb) Multiome | 164 |
Genomic view of the CHAF1A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.