Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Transcription factors with Perturb-seq knockdown data for BCAR3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BCAR3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BCAR3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:93,447,560–93,449,029 | 233.7 kb | Distal (>10kb) Multiome | 726 | |
| chr1:93,474,058–93,474,810 | 207.5 kb | Distal (>10kb) Multiome | 63 | |
| chr1:93,484,155–93,485,250 | 197.0 kb | Distal (>10kb) Multiome | 146 | |
| chr1:93,622,085–93,622,775 | 59.5 kb | Distal (>10kb) Multiome | 282 | |
| chr1:93,678,776–93,679,511 | 2.6 kb | Proximal (<10kb) Multiome | 96 | |
| chr1:93,679,668–93,679,844 | 2.0 kb | Proximal (<10kb) | 33 | |
| chr1:93,680,202–93,680,638 | 1.2 kb | Proximal (<10kb) | 174 | |
| chr1:93,680,733–93,682,277 | 85 bp | At TSS Multiome | 717 | |
| chr1:93,691,208–93,691,662 | 9.4 kb | Proximal (<10kb) | 57 | |
| chr1:93,814,055–93,816,005 | 133.5 kb | Distal (>10kb) Multiome HiCAR | 533 | |
| chr1:93,820,560–93,821,453 | 139.3 kb | Distal (>10kb) Multiome HiCAR | 160 | |
| chr1:93,845,985–93,846,892 | 164.4 kb | Distal (>10kb) Multiome HiCAR | 732 | |
| chr1:93,846,995–93,848,315 | 165.7 kb | Distal (>10kb) Multiome HiCAR | 954 | |
| chr1:93,878,726–93,879,400 | 197.4 kb | Distal (>10kb) Multiome | 851 | |
| chr1:93,908,647–93,910,233 | 227.8 kb | Distal (>10kb) Multiome | 901 |
Genomic view of the BCAR3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.