The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:46,903,672–46,904,257 | 286.9 kb | Distal (>10kb) Multiome | 417 | |
| chrX:46,912,095–46,913,434 | 278.3 kb | Distal (>10kb) Multiome | 558 | |
| chrX:47,129,095–47,129,667 | 61.5 kb | Distal (>10kb) Multiome | 538 | |
| chrX:47,144,621–47,145,590 | 45.8 kb | Distal (>10kb) Multiome | 649 | |
| chrX:47,190,608–47,191,059 | 132 bp | At TSS Multiome | 536 | |
| chrX:47,191,642–47,192,226 | 1.2 kb | Proximal (<10kb) Multiome | 138 | |
| chrX:47,193,067–47,193,943 | 2.9 kb | Proximal (<10kb) Multiome | 651 | |
| chrX:47,197,196–47,197,435 | 6.3 kb | Proximal (<10kb) | 26 | |
| chrX:47,198,176–47,198,654 | 7.3 kb | Proximal (<10kb) | 34 | |
| chrX:47,217,449–47,219,337 | 27.2 kb | Distal (>10kb) Multiome | 736 | |
| chrX:47,232,860–47,233,865 | 42.7 kb | Distal (>10kb) Multiome | 684 | |
| chrX:47,321,409–47,321,883 | 130.8 kb | Distal (>10kb) Multiome | 127 | |
| chrX:47,361,756–47,362,456 | 171.3 kb | Distal (>10kb) Multiome | 297 | |
| chrX:47,381,691–47,382,470 | 191.3 kb | Distal (>10kb) Multiome HiCAR | 17 | |
| chrX:47,482,474–47,483,311 | 291.9 kb | Distal (>10kb) Multiome | 573 | |
| chrX:47,523,425–47,523,870 | 332.7 kb | Distal (>10kb) Multiome HiCAR | 403 |
Genomic view of the UBA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.