Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2015]
Transcription factors with Perturb-seq knockdown data for ST8SIA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ST8SIA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ST8SIA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:21,656,935–21,658,449 | 676.8 kb | Distal (>10kb) Multiome HiCAR | 715 | |
| chr12:22,045,900–22,047,085 | 288.3 kb | Distal (>10kb) Multiome HiCAR | 738 | |
| chr12:22,245,670–22,246,345 | 88.6 kb | Distal (>10kb) Multiome HiCAR | 45 | |
| chr12:22,250,634–22,251,163 | at TSS | At TSS | 96 | |
| chr12:22,254,388–22,254,791 | 3.6 kb | Proximal (<10kb) | 10 | |
| chr12:22,255,112–22,255,476 | 4.3 kb | Proximal (<10kb) | 49 | |
| chr12:22,333,730–22,336,157 | 853 bp | At TSS Multiome HiCAR | 600 | |
| chr12:22,340,086–22,340,871 | 5.4 kb | Proximal (<10kb) | 33 | |
| chr12:22,353,447–22,354,267 | 19.2 kb | Distal (>10kb) Multiome | 141 | |
| chr12:22,376,592–22,377,286 | 42.3 kb | Distal (>10kb) Multiome | 149 | |
| chr12:22,378,068–22,378,655 | 1.1 kb | Proximal (<10kb) | 59 | |
| chr12:22,436,588–22,437,692 | 102.5 kb | Distal (>10kb) Multiome HiCAR | 80 | |
| chr12:22,438,546–22,438,957 | 1.5 kb | Proximal (<10kb) | 7 | |
| chr12:22,443,250–22,443,763 | 108.8 kb | Distal (>10kb) Multiome HiCAR | 286 | |
| chr12:22,543,368–22,545,088 | 209.8 kb | Distal (>10kb) Multiome | 1040 | |
| chr12:22,624,339–22,626,265 | 290.4 kb | Distal (>10kb) Multiome | 1049 | |
| chr12:22,699,795–22,700,629 | 365.2 kb | Distal (>10kb) Multiome | 93 | |
| chr12:22,700,769–22,701,762 | 366.6 kb | Distal (>10kb) Multiome | 149 |
Genomic view of the ST8SIA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.