Enables aspartic endopeptidase activity, intramembrane cleaving and protein homodimerization activity. Involved in T cell receptor signaling pathway; membrane protein proteolysis; and positive regulation of calcineurin-NFAT signaling cascade. Located in Golgi-associated vesicle membrane; endoplasmic reticulum; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for SPPL3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPPL3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPPL3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:120,640,005–120,641,704 | 263.9 kb | Distal (>10kb) Multiome | 562 | |
| chr12:120,648,914–120,649,665 | 255.1 kb | Distal (>10kb) Multiome | 405 | |
| chr12:120,668,575–120,669,135 | 235.5 kb | Distal (>10kb) Multiome | 98 | |
| chr12:120,686,185–120,687,741 | 217.6 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:120,710,025–120,711,277 | 194.0 kb | Distal (>10kb) Multiome | 573 | |
| chr12:120,725,271–120,726,309 | 178.5 kb | Distal (>10kb) Multiome | 663 | |
| chr12:120,822,842–120,823,717 | 81.2 kb | Distal (>10kb) Multiome | 117 | |
| chr12:120,903,538–120,904,786 | 45 bp | At TSS Multiome | 749 | |
| chr12:121,016,022–121,016,705 | 112.1 kb | Distal (>10kb) Multiome | 674 | |
| chr12:121,026,128–121,026,637 | 122.1 kb | Distal (>10kb) Multiome | 253 |
Genomic view of the SPPL3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.