This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
Transcription factors with Perturb-seq knockdown data for SPARC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = SPARC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of SPARC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:151,405,271–151,406,618 | 281.3 kb | Distal (>10kb) Multiome | 175 | |
| chr5:151,447,360–151,448,441 | 239.3 kb | Distal (>10kb) Multiome | 900 | |
| chr5:151,496,053–151,496,694 | 190.6 kb | Distal (>10kb) Multiome | 64 | |
| chr5:151,500,606–151,501,090 | 186.0 kb | Distal (>10kb) Multiome | 332 | |
| chr5:151,590,936–151,591,543 | 95.6 kb | Distal (>10kb) Multiome | 83 | |
| chr5:151,658,968–151,659,631 | 27.8 kb | Distal (>10kb) Multiome | 335 | |
| chr5:151,673,039–151,674,114 | 13.3 kb | Distal (>10kb) Multiome | 193 | |
| chr5:151,684,706–151,685,487 | 1.9 kb | Proximal (<10kb) Multiome | 323 | |
| chr5:151,686,404–151,687,445 | 70 bp | At TSS Multiome | 314 | |
| chr5:151,696,457–151,697,030 | 9.5 kb | Proximal (<10kb) | 137 | |
| chr5:151,740,170–151,741,128 | 53.7 kb | Distal (>10kb) Multiome | 315 | |
| chr5:151,757,903–151,759,332 | 71.8 kb | Distal (>10kb) Multiome | 766 | |
| chr5:151,770,363–151,773,120 | 84.8 kb | Distal (>10kb) Multiome | 1055 | |
| chr5:151,902,932–151,903,823 | 216.5 kb | Distal (>10kb) Multiome | 181 |
Genomic view of the SPARC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.