Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for RPS6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = RPS6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of RPS6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:19,102,191–19,103,414 | 277.3 kb | Distal (>10kb) Multiome | 770 | |
| chr9:19,126,915–19,128,564 | 252.7 kb | Distal (>10kb) Multiome | 808 | |
| chr9:19,201,888–19,203,074 | 177.8 kb | Distal (>10kb) Multiome | 569 | |
| chr9:19,229,553–19,231,458 | 149.7 kb | Distal (>10kb) Multiome | 973 | |
| chr9:19,372,546–19,372,968 | 7.3 kb | Proximal (<10kb) | 44 | |
| chr9:19,374,591–19,374,888 | 5.3 kb | Proximal (<10kb) | 118 | |
| chr9:19,375,054–19,375,272 | 5.0 kb | Proximal (<10kb) | 81 | |
| chr9:19,379,591–19,380,878 | 50 bp | At TSS Multiome | 1052 | |
| chr9:19,408,145–19,409,950 | 28.7 kb | Distal (>10kb) Multiome | 773 | |
| chr9:19,446,288–19,446,838 | 66.4 kb | Distal (>10kb) Multiome HiCAR | 110 | |
| chr9:19,464,477–19,465,453 | 84.7 kb | Distal (>10kb) Multiome | 427 |
Genomic view of the RPS6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.