Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
Transcription factors with Perturb-seq knockdown data for PRKG1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKG1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKG1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:50,787,771–50,788,455 | 286.4 kb | Distal (>10kb) Multiome | 88 | |
| chr10:51,073,446–51,075,055 | 103 bp | At TSS Multiome | 475 | |
| chr10:51,075,579–51,075,750 | 1.3 kb | Proximal (<10kb) | 12 | |
| chr10:51,079,439–51,079,610 | 5.1 kb | Proximal (<10kb) | 23 | |
| chr10:51,083,422–51,083,922 | 9.1 kb | Proximal (<10kb) | 86 | |
| chr10:51,268,102–51,268,601 | 193.8 kb | Distal (>10kb) Multiome | 94 | |
| chr10:51,914,936–51,915,602 | 840.8 kb | Distal (>10kb) Multiome | 160 | |
| chr10:51,918,559–51,919,423 | 844.6 kb | Distal (>10kb) Multiome | 148 | |
| chr10:52,039,217–52,039,988 | 965.0 kb | Distal (>10kb) Multiome HiCAR | 116 | |
| chr10:52,128,116–52,128,321 | 23 bp | At TSS | 29 | |
| chr10:52,308,085–52,309,123 | 1234.1 kb | Distal (>10kb) Multiome | 588 | |
| chr10:52,312,997–52,315,411 | 1239.8 kb | Distal (>10kb) Multiome | 866 |
Genomic view of the PRKG1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.