NDUFS3

NADH:ubiquinone oxidoreductase core subunit S3 | CI-30

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]

Member of: DE-1 DE-1.40 Developmental clusters: GC4

Biological processes 28 terms

NADH dehydrogenase (ubiquinone) activity (GO:0008137)NADH dehydrogenase (ubiquinone) activity (GO:0008137)NADH dehydrogenase (ubiquinone) activity (GO:0008137)NADH dehydrogenase (ubiquinone) activity (GO:0008137)NADH dehydrogenase activity (GO:0003954)aerobic respiration (GO:0009060)electron transfer activity (GO:0009055)mitochondrial electron transport, NADH to ubiquinone (GO:0006120)mitochondrial electron transport, NADH to ubiquinone (GO:0006120)mitochondrial electron transport, NADH to ubiquinone (GO:0006120)mitochondrial inner membrane (GO:0005743)mitochondrial inner membrane (GO:0005743)mitochondrial matrix (GO:0005759)mitochondrial membrane (GO:0031966)mitochondrial respiratory chain complex I assembly (GO:0032981)mitochondrion (GO:0005739)mitochondrion (GO:0005739)mitochondrion (GO:0005739)oxidoreductase activity, acting on NAD(P)H (GO:0016651)protein binding (GO:0005515)proton motive force-driven mitochondrial ATP synthesis (GO:0042776)proton transmembrane transport (GO:1902600)reactive oxygen species metabolic process (GO:0072593)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)substantia nigra development (GO:0021762)

Expression (TPM)

TFs regulating NDUFS3 0 TFs

Transcription factors with Perturb-seq knockdown data for NDUFS3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFS3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to NDUFS3

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFS3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr11:47,354,896–47,355,720	223.7 kb	Distal (>10kb) Multiome	670
chr11:47,399,791–47,400,763	178.8 kb	Distal (>10kb) Multiome	450
chr11:47,407,671–47,409,059	170.7 kb	Distal (>10kb) Multiome	986
chr11:47,425,998–47,427,263	152.6 kb	Distal (>10kb) Multiome	879
chr11:47,508,005–47,509,036	70.5 kb	Distal (>10kb) Multiome	73
chr11:47,552,255–47,553,700	25.8 kb	Distal (>10kb) Multiome	834
chr11:47,564,988–47,566,330	13.5 kb	Distal (>10kb) Multiome	842
chr11:47,578,427–47,579,510	124 bp	At TSS Multiome	1080
chr11:47,589,692–47,590,847	11.3 kb	Distal (>10kb) Multiome	161
chr11:47,594,122–47,594,733	15.3 kb	Distal (>10kb) Multiome	153
chr11:47,598,993–47,599,885	20.3 kb	Distal (>10kb) Multiome	67
chr11:47,642,000–47,643,006	63.5 kb	Distal (>10kb) Multiome	973
chr11:47,714,892–47,715,563	136.3 kb	Distal (>10kb) Multiome	607
chr11:47,766,463–47,768,105	188.4 kb	Distal (>10kb) Multiome	961
chr11:47,847,845–47,848,997	269.4 kb	Distal (>10kb) Multiome	863

Genome Browser

Genomic view of the NDUFS3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.