The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for NCOA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NCOA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NCOA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:47,319,319–47,320,069 | 182.1 kb | Distal (>10kb) Multiome | 412 | |
| chr20:47,348,216–47,349,067 | 153.3 kb | Distal (>10kb) Multiome | 230 | |
| chr20:47,352,182–47,352,868 | 149.4 kb | Distal (>10kb) Multiome | 352 | |
| chr20:47,356,649–47,357,177 | 145.1 kb | Distal (>10kb) Multiome | 667 | |
| chr20:47,357,312–47,358,028 | 144.1 kb | Distal (>10kb) Multiome | 996 | |
| chr20:47,383,734–47,384,319 | 117.8 kb | Distal (>10kb) Multiome HiCAR | 786 | |
| chr20:47,501,324–47,502,272 | 3 bp | At TSS Multiome | 1052 | |
| chr20:47,761,548–47,762,222 | 259.9 kb | Distal (>10kb) Multiome | 87 | |
| chr20:47,785,376–47,786,125 | 283.7 kb | Distal (>10kb) Multiome | 423 | |
| chr20:47,862,561–47,863,436 | 361.1 kb | Distal (>10kb) Multiome | 89 | |
| chr20:47,876,376–47,876,884 | 374.7 kb | Distal (>10kb) Multiome | 89 |
Genomic view of the NCOA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.