Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPS15. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPS15 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPS15, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:36,166,478–36,167,190 | 297.6 kb | Distal (>10kb) Multiome | 85 | |
| chr1:36,176,148–36,176,823 | 287.9 kb | Distal (>10kb) Multiome | 141 | |
| chr1:36,178,119–36,179,461 | 285.4 kb | Distal (>10kb) Multiome | 222 | |
| chr1:36,187,827–36,188,464 | 276.2 kb | Distal (>10kb) Multiome | 572 | |
| chr1:36,223,459–36,225,047 | 240.1 kb | Distal (>10kb) Multiome | 1017 | |
| chr1:36,305,654–36,307,599 | 157.4 kb | Distal (>10kb) Multiome | 826 | |
| chr1:36,320,781–36,321,934 | 143.0 kb | Distal (>10kb) Multiome | 522 | |
| chr1:36,322,032–36,324,527 | 140.6 kb | Distal (>10kb) Multiome | 699 | |
| chr1:36,384,603–36,387,142 | 78.3 kb | Distal (>10kb) Multiome | 1050 | |
| chr1:36,387,290–36,388,127 | 76.6 kb | Distal (>10kb) Multiome | 280 | |
| chr1:36,397,267–36,398,140 | 66.5 kb | Distal (>10kb) Multiome | 787 | |
| chr1:36,400,687–36,401,905 | 63.0 kb | Distal (>10kb) Multiome | 721 | |
| chr1:36,449,991–36,450,940 | 13.8 kb | Distal (>10kb) Multiome | 725 | |
| chr1:36,456,615–36,456,855 | 7.5 kb | Proximal (<10kb) | 32 | |
| chr1:36,463,701–36,464,970 | 70 bp | At TSS Multiome | 1027 | |
| chr1:36,465,219–36,465,457 | 835 bp | At TSS | 79 | |
| chr1:36,481,699–36,482,274 | 17.7 kb | Distal (>10kb) Multiome | 133 | |
| chr1:36,736,272–36,736,888 | 272.2 kb | Distal (>10kb) Multiome | 32 |
Genomic view of the MRPS15 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.