This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]
Transcription factors with Perturb-seq knockdown data for MKRN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MKRN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MKRN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:140,229,607–140,231,952 | 249.4 kb | Distal (>10kb) Multiome | 914 | |
| chr7:140,241,793–140,243,015 | 237.3 kb | Distal (>10kb) Multiome | 460 | |
| chr7:140,278,313–140,279,187 | 200.7 kb | Distal (>10kb) Multiome | 65 | |
| chr7:140,397,976–140,398,715 | 81.0 kb | Distal (>10kb) Multiome | 696 | |
| chr7:140,403,831–140,404,498 | 75.4 kb | Distal (>10kb) Multiome | 333 | |
| chr7:140,455,562–140,456,050 | 23.6 kb | Distal (>10kb) Multiome | 50 | |
| chr7:140,478,495–140,480,078 | 26 bp | At TSS Multiome | 869 | |
| chr7:140,481,148–140,482,022 | 2.1 kb | Proximal (<10kb) Multiome | 15 | |
| chr7:140,484,537–140,485,185 | 6.0 kb | Proximal (<10kb) | 82 | |
| chr7:140,488,120–140,488,789 | 9.0 kb | Proximal (<10kb) Multiome | 230 | |
| chr7:140,517,960–140,518,418 | 38.7 kb | Distal (>10kb) Multiome | 472 | |
| chr7:140,541,306–140,541,982 | 62.3 kb | Distal (>10kb) Multiome | 422 | |
| chr7:140,639,882–140,641,732 | 161.3 kb | Distal (>10kb) Multiome HiCAR | 486 | |
| chr7:140,672,673–140,674,052 | 193.5 kb | Distal (>10kb) Multiome | 648 | |
| chr7:140,696,318–140,697,705 | 217.3 kb | Distal (>10kb) Multiome | 1002 |
Genomic view of the MKRN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.