This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for LEFTY1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LEFTY1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LEFTY1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:225,627,340–225,627,898 | 283.8 kb | Distal (>10kb) Multiome | 254 | |
| chr1:225,652,334–225,654,131 | 258.1 kb | Distal (>10kb) Multiome | 786 | |
| chr1:225,754,870–225,755,548 | 156.3 kb | Distal (>10kb) Multiome | 382 | |
| chr1:225,768,775–225,769,570 | 142.2 kb | Distal (>10kb) Multiome | 313 | |
| chr1:225,771,884–225,772,638 | 139.0 kb | Distal (>10kb) Multiome | 488 | |
| chr1:225,777,167–225,778,412 | 133.6 kb | Distal (>10kb) Multiome | 942 | |
| chr1:225,809,711–225,811,548 | 101.2 kb | Distal (>10kb) Multiome | 654 | |
| chr1:225,845,424–225,846,556 | 65.4 kb | Distal (>10kb) Multiome | 416 | |
| chr1:225,881,255–225,884,173 | 29.0 kb | Distal (>10kb) Multiome HiCAR | 879 | |
| chr1:225,887,561–225,887,851 | 1.3 kb | Proximal (<10kb) | 49 | |
| chr1:225,888,552–225,889,596 | 22.2 kb | Distal (>10kb) Multiome HiCAR | 185 | |
| chr1:225,898,735–225,899,922 | 11.9 kb | Distal (>10kb) Multiome | 335 | |
| chr1:225,903,782–225,904,454 | 7.4 kb | Proximal (<10kb) Multiome | 40 | |
| chr1:225,908,825–225,909,327 | 2.4 kb | Proximal (<10kb) Multiome | 101 | |
| chr1:225,910,978–225,911,799 | 5 bp | At TSS Multiome | 344 | |
| chr1:225,923,776–225,924,773 | 13.0 kb | Distal (>10kb) Multiome | 960 | |
| chr1:225,939,444–225,939,998 | 28.3 kb | Distal (>10kb) Multiome | 62 | |
| chr1:225,945,518–225,946,038 | 34.4 kb | Distal (>10kb) Multiome | 172 | |
| chr1:225,948,616–225,949,435 | 37.7 kb | Distal (>10kb) Multiome | 175 | |
| chr1:225,957,776–225,958,797 | 47.0 kb | Distal (>10kb) Multiome | 175 | |
| chr1:225,999,043–226,000,159 | 88.1 kb | Distal (>10kb) Multiome | 969 | |
| chr1:226,061,587–226,063,333 | 151.1 kb | Distal (>10kb) Multiome | 976 | |
| chr1:226,082,913–226,084,135 | 172.2 kb | Distal (>10kb) Multiome | 852 | |
| chr1:226,109,567–226,111,083 | 198.7 kb | Distal (>10kb) Multiome | 768 | |
| chr1:226,121,192–226,122,943 | 210.5 kb | Distal (>10kb) Multiome | 840 | |
| chr1:226,125,045–226,125,680 | 214.0 kb | Distal (>10kb) Multiome | 373 | |
| chr1:226,186,167–226,187,132 | 275.4 kb | Distal (>10kb) Multiome | 939 | |
| chr1:226,210,358–226,211,556 | 299.5 kb | Distal (>10kb) Multiome | 262 |
Genomic view of the LEFTY1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.