Enables ubiquitin protein ligase activity. Involved in several processes, including negative regulation of HRI-mediated signaling; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein polyubiquitination. Located in cytosol. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for KCMF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KCMF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KCMF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:84,855,538–84,856,452 | 115.1 kb | Distal (>10kb) Multiome | 137 | |
| chr2:84,879,622–84,881,453 | 90.1 kb | Distal (>10kb) Multiome | 434 | |
| chr2:84,881,776–84,882,617 | 88.8 kb | Distal (>10kb) Multiome | 438 | |
| chr2:84,904,641–84,908,593 | 63.7 kb | Distal (>10kb) Multiome | 1075 | |
| chr2:84,925,524–84,927,306 | 45.1 kb | Distal (>10kb) Multiome | 1034 | |
| chr2:84,957,753–84,958,825 | 12.9 kb | Distal (>10kb) Multiome | 500 | |
| chr2:84,959,740–84,960,486 | 11.0 kb | Distal (>10kb) Multiome | 381 | |
| chr2:84,969,143–84,969,506 | 1.6 kb | Proximal (<10kb) | 109 | |
| chr2:84,970,310–84,972,229 | 110 bp | At TSS Multiome | 809 | |
| chr2:84,986,815–84,987,471 | 16.0 kb | Distal (>10kb) Multiome | 29 | |
| chr2:85,033,412–85,033,982 | 62.5 kb | Distal (>10kb) Multiome | 100 | |
| chr2:85,066,689–85,067,387 | 96.0 kb | Distal (>10kb) Multiome | 352 | |
| chr2:85,119,847–85,120,292 | 149.0 kb | Distal (>10kb) Multiome | 127 | |
| chr2:85,132,493–85,135,747 | 162.1 kb | Distal (>10kb) Multiome | 660 | |
| chr2:85,174,805–85,176,312 | 204.5 kb | Distal (>10kb) Multiome | 399 | |
| chr2:85,178,933–85,179,785 | 208.2 kb | Distal (>10kb) Multiome | 95 | |
| chr2:85,237,653–85,238,609 | 267.0 kb | Distal (>10kb) Multiome | 190 | |
| chr2:85,255,366–85,256,615 | 285.1 kb | Distal (>10kb) Multiome | 414 |
Genomic view of the KCMF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.