Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in cell development; neuron differentiation; and regulation of DNA-templated transcription. Predicted to act upstream of or within heart development. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2025]
Transcription factors with Perturb-seq knockdown data for IRX4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IRX4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IRX4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:1,593,846–1,595,021 | 288.2 kb | Distal (>10kb) Multiome | 682 | |
| chr5:1,633,700–1,634,325 | 248.7 kb | Distal (>10kb) Multiome | 745 | |
| chr5:1,644,948–1,645,570 | 237.6 kb | Distal (>10kb) Multiome | 437 | |
| chr5:1,666,897–1,668,526 | 215.4 kb | Distal (>10kb) Multiome | 301 | |
| chr5:1,799,167–1,800,245 | 82.9 kb | Distal (>10kb) Multiome | 744 | |
| chr5:1,800,343–1,801,861 | 81.3 kb | Distal (>10kb) Multiome | 895 | |
| chr5:1,827,805–1,828,459 | 54.6 kb | Distal (>10kb) Multiome | 84 | |
| chr5:1,867,435–1,868,302 | 15.1 kb | Distal (>10kb) Multiome | 169 | |
| chr5:1,874,486–1,877,084 | 7.8 kb | Proximal (<10kb) Multiome | 194 | |
| chr5:1,877,802–1,878,575 | 4.2 kb | Proximal (<10kb) | 71 | |
| chr5:1,880,966–1,883,137 | 134 bp | At TSS Multiome | 459 | |
| chr5:1,883,292–1,884,343 | 1.1 kb | Proximal (<10kb) Multiome | 279 | |
| chr5:1,884,792–1,888,153 | 4.5 kb | Proximal (<10kb) Multiome | 186 | |
| chr5:1,894,261–1,894,531 | 7.3 kb | Proximal (<10kb) | 14 | |
| chr5:1,930,629–1,931,989 | 48.3 kb | Distal (>10kb) Multiome | 233 | |
| chr5:2,038,286–2,039,379 | 156.0 kb | Distal (>10kb) Multiome | 517 |
Genomic view of the IRX4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.