Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
Transcription factors with Perturb-seq knockdown data for IBTK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IBTK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IBTK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:82,240,251–82,240,497 | 7.3 kb | Proximal (<10kb) | 29 | |
| chr6:82,246,942–82,248,798 | 17 bp | At TSS Multiome | 957 | |
| chr6:82,249,708–82,250,214 | 2.0 kb | Proximal (<10kb) | 80 | |
| chr6:82,251,223–82,251,913 | 3.5 kb | Proximal (<10kb) | 13 | |
| chr6:82,252,022–82,252,212 | 4.3 kb | Proximal (<10kb) | 48 | |
| chr6:82,256,786–82,258,089 | 9.6 kb | Proximal (<10kb) Multiome | 171 | |
| chr6:82,363,222–82,366,030 | 116.1 kb | Distal (>10kb) Multiome | 857 | |
| chr6:82,607,708–82,609,057 | 360.5 kb | Distal (>10kb) Multiome HiCAR | 138 | |
| chr6:83,065,409–83,066,246 | 818.1 kb | Distal (>10kb) Multiome HiCAR | 863 | |
| chr6:83,067,273–83,069,447 | 820.2 kb | Distal (>10kb) Multiome HiCAR | 1006 | |
| chr6:83,192,782–83,194,476 | 945.6 kb | Distal (>10kb) Multiome HiCAR | 948 |
Genomic view of the IBTK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.