GOT1
glutamic-oxaloacetic transaminase 1 | AST, AST1, SGOT

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Member of: DE-11 Developmental clusters: GC3
Biological processes 51 terms
(2S)-2-aminobutanoate:2-oxoglutarate transaminase activity (GO:0120554)(2S)-2-aminobutanoate:2-oxoglutarate transaminase activity (GO:0120554)2-oxoglutarate metabolic process (GO:0006103)L-aspartate biosynthetic process (GO:0006532)L-aspartate catabolic process (GO:0006533)L-aspartate catabolic process (GO:0006533)L-aspartate:2-oxoglutarate transaminase activity (GO:0004069)L-aspartate:2-oxoglutarate transaminase activity (GO:0004069)L-aspartate:2-oxoglutarate transaminase activity (GO:0004069)L-aspartate:2-oxoglutarate transaminase activity (GO:0004069)L-cysteine:2-oxoglutarate transaminase activity (GO:0047801)L-cysteine:2-oxoglutarate transaminase activity (GO:0047801)L-glutamate biosynthetic process (GO:0097054)amino acid metabolic process (GO:0006520)aspartate metabolic process (GO:0006531)axon terminus (GO:0043679)carboxylic acid binding (GO:0031406)catalytic activity (GO:0003824)cellular response to cAMP (GO:0071320)cellular response to glucagon stimulus (GO:0071377)cellular response to insulin stimulus (GO:0032869)cellular response to mechanical stimulus (GO:0071260)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)dicarboxylic acid metabolic process (GO:0043648)extracellular exosome (GO:0070062)gluconeogenesis (GO:0006094)glutamate metabolic process (GO:0006536)glycerol biosynthetic process (GO:0006114)glycerol biosynthetic process (GO:0006114)malate-aspartate shuttle (GO:0043490)malate-aspartate shuttle (GO:0043490)negative regulation of collagen biosynthetic process (GO:0032966)negative regulation of cytosolic calcium ion concentration (GO:0051481)negative regulation of mitochondrial depolarization (GO:0051902)nucleus (GO:0005634)oxaloacetate metabolic process (GO:0006107)phosphatidylserine decarboxylase activity (GO:0004609)positive regulation of transforming growth factor beta receptor signaling pathway (GO:0030511)protein binding (GO:0005515)pyridoxal phosphate binding (GO:0030170)response to cadmium ion (GO:0046686)response to carbohydrate (GO:0009743)response to glucocorticoid (GO:0051384)response to immobilization stress (GO:0035902)response to transition metal nanoparticle (GO:1990267)transaminase activity (GO:0008483)transdifferentiation (GO:0060290)
Expression (TPM)
GOT1 — as a Regulated Gene

TFs regulating GOT1 0 TFs

Transcription factors with Perturb-seq knockdown data for GOT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GOT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to GOT1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GOT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr10:99,232,004–99,233,058 197.9 kb Distal (>10kb) Multiome 155
chr10:99,236,018–99,237,092 194.4 kb Distal (>10kb) Multiome 141
chr10:99,266,221–99,266,995 164.0 kb Distal (>10kb) Multiome 113
chr10:99,357,908–99,359,626 71.5 kb Distal (>10kb) Multiome 160
chr10:99,370,159–99,370,697 60.2 kb Distal (>10kb) Multiome 14
chr10:99,430,207–99,431,536 184 bp At TSS Multiome 882
chr10:99,519,844–99,520,769 89.6 kb Distal (>10kb) Multiome HiCAR 331
chr10:99,522,567–99,524,013 92.7 kb Distal (>10kb) Multiome HiCAR 201
chr10:99,527,131–99,527,911 96.9 kb Distal (>10kb) Multiome 89
chr10:99,532,575–99,533,287 102.3 kb Distal (>10kb) Multiome 160
chr10:99,534,863–99,536,063 104.8 kb Distal (>10kb) Multiome 193
chr10:99,536,574–99,538,002 106.4 kb Distal (>10kb) Multiome 279
chr10:99,619,825–99,621,725 190.1 kb Distal (>10kb) Multiome 1070
chr10:99,658,847–99,660,052 228.8 kb Distal (>10kb) Multiome 728

Genome Browser

Genomic view of the GOT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr10:99,222,004 – 99,670,052
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq