Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]
Transcription factors with Perturb-seq knockdown data for GLDC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GLDC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GLDC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:6,364,728–6,365,487 | 280.6 kb | Distal (>10kb) Multiome | 58 | |
| chr9:6,411,893–6,414,477 | 232.6 kb | Distal (>10kb) Multiome | 913 | |
| chr9:6,590,688–6,591,679 | 54.6 kb | Distal (>10kb) Multiome | 403 | |
| chr9:6,591,883–6,592,036 | 6.8 kb | Proximal (<10kb) | 17 | |
| chr9:6,592,227–6,592,641 | 53.2 kb | Distal (>10kb) Multiome | 82 | |
| chr9:6,645,085–6,646,200 | 28 bp | At TSS Multiome | 656 | |
| chr9:6,648,431–6,648,592 | 2.7 kb | Proximal (<10kb) | 48 | |
| chr9:6,680,771–6,682,229 | 35.8 kb | Distal (>10kb) Multiome | 1070 | |
| chr9:6,692,445–6,693,412 | 47.2 kb | Distal (>10kb) Multiome | 196 | |
| chr9:6,703,765–6,704,853 | 58.6 kb | Distal (>10kb) Multiome | 655 | |
| chr9:6,715,763–6,716,690 | 70.4 kb | Distal (>10kb) Multiome | 613 | |
| chr9:6,757,043–6,759,285 | 112.1 kb | Distal (>10kb) Multiome | 1099 | |
| chr9:6,771,611–6,772,338 | 126.2 kb | Distal (>10kb) Multiome | 180 | |
| chr9:6,776,647–6,777,775 | 131.4 kb | Distal (>10kb) Multiome | 152 | |
| chr9:6,779,316–6,780,772 | 134.1 kb | Distal (>10kb) Multiome | 389 | |
| chr9:6,788,081–6,789,168 | 143.0 kb | Distal (>10kb) Multiome | 445 |
Genomic view of the GLDC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.