This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for G3BP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = G3BP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of G3BP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:151,496,053–151,496,694 | 275.6 kb | Distal (>10kb) Multiome | 64 | |
| chr5:151,500,606–151,501,090 | 271.0 kb | Distal (>10kb) Multiome | 332 | |
| chr5:151,590,936–151,591,543 | 180.6 kb | Distal (>10kb) Multiome | 83 | |
| chr5:151,658,968–151,659,631 | 112.8 kb | Distal (>10kb) Multiome | 335 | |
| chr5:151,673,039–151,674,114 | 98.3 kb | Distal (>10kb) Multiome | 193 | |
| chr5:151,684,706–151,685,487 | 86.9 kb | Distal (>10kb) Multiome | 323 | |
| chr5:151,686,404–151,687,445 | 84.9 kb | Distal (>10kb) Multiome | 314 | |
| chr5:151,740,170–151,741,128 | 31.3 kb | Distal (>10kb) Multiome | 315 | |
| chr5:151,757,903–151,759,332 | 13.2 kb | Distal (>10kb) Multiome | 766 | |
| chr5:151,770,363–151,773,120 | 252 bp | At TSS Multiome | 1055 | |
| chr5:151,780,481–151,780,842 | 8.6 kb | Proximal (<10kb) | 35 | |
| chr5:151,902,932–151,903,823 | 131.5 kb | Distal (>10kb) Multiome | 181 | |
| chr5:152,051,788–152,052,791 | 280.2 kb | Distal (>10kb) Multiome | 165 | |
| chr5:152,306,139–152,307,232 | 534.8 kb | Distal (>10kb) Multiome HiCAR | 80 | |
| chr5:152,310,015–152,310,503 | 538.3 kb | Distal (>10kb) Multiome HiCAR | 69 | |
| chr5:152,464,087–152,464,718 | 692.4 kb | Distal (>10kb) Multiome HiCAR | 17 |
Genomic view of the G3BP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.