Transcription factors with Perturb-seq knockdown data for FAM228B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM228B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM228B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:23,925,864–23,928,023 | 149.8 kb | Distal (>10kb) Multiome | 1102 | |
| chr2:23,939,310–23,941,043 | 136.4 kb | Distal (>10kb) Multiome | 856 | |
| chr2:24,009,633–24,010,873 | 66.7 kb | Distal (>10kb) Multiome | 382 | |
| chr2:24,046,941–24,048,071 | 29.4 kb | Distal (>10kb) Multiome | 719 | |
| chr2:24,049,244–24,050,644 | 27.2 kb | Distal (>10kb) Multiome | 554 | |
| chr2:24,075,859–24,077,721 | 252 bp | At TSS Multiome | 1100 | |
| chr2:24,083,513–24,085,355 | 7.5 kb | Proximal (<10kb) Multiome | 1088 | |
| chr2:24,123,015–24,123,986 | 46.6 kb | Distal (>10kb) Multiome | 899 | |
| chr2:24,191,824–24,192,305 | 115.1 kb | Distal (>10kb) Multiome | 68 | |
| chr2:24,280,210–24,280,709 | 203.4 kb | Distal (>10kb) Multiome | 38 | |
| chr2:24,315,559–24,316,125 | 238.8 kb | Distal (>10kb) Multiome | 99 | |
| chr2:24,359,764–24,361,015 | 283.6 kb | Distal (>10kb) Multiome | 909 |
Genomic view of the FAM228B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.