This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for EOMES. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EOMES upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EOMES, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:27,483,148–27,485,398 | 237.8 kb | Distal (>10kb) Multiome | 917 | |
| chr3:27,632,489–27,633,437 | 89.3 kb | Distal (>10kb) Multiome | 378 | |
| chr3:27,711,660–27,716,254 | 8.2 kb | Proximal (<10kb) Multiome | 783 | |
| chr3:27,717,292–27,717,729 | 5.0 kb | Proximal (<10kb) | 17 | |
| chr3:27,719,373–27,720,284 | 2.4 kb | Proximal (<10kb) | 46 | |
| chr3:27,720,744–27,724,896 | 462 bp | At TSS Multiome | 708 | |
| chr3:27,727,875–27,731,045 | 8.1 kb | Proximal (<10kb) Multiome | 446 | |
| chr3:27,731,146–27,731,868 | 8.4 kb | Proximal (<10kb) | 218 | |
| chr3:28,240,444–28,242,372 | 519.3 kb | Distal (>10kb) Multiome HiCAR | 1004 | |
| chr3:28,348,014–28,349,873 | 626.7 kb | Distal (>10kb) Multiome HiCAR | 1118 |
Genomic view of the EOMES locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.