The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
Transcription factors with Perturb-seq knockdown data for EIF4G1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF4G1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF4G1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:184,017,103–184,018,456 | 296.6 kb | Distal (>10kb) Multiome | 878 | |
| chr3:184,134,086–184,134,568 | 180.4 kb | Distal (>10kb) Multiome | 732 | |
| chr3:184,134,801–184,135,887 | 179.2 kb | Distal (>10kb) Multiome | 941 | |
| chr3:184,154,967–184,155,880 | 159.2 kb | Distal (>10kb) Multiome | 580 | |
| chr3:184,174,432–184,175,524 | 139.8 kb | Distal (>10kb) Multiome | 765 | |
| chr3:184,176,129–184,177,706 | 137.8 kb | Distal (>10kb) Multiome | 480 | |
| chr3:184,185,411–184,187,065 | 128.6 kb | Distal (>10kb) Multiome | 878 | |
| chr3:184,229,229–184,229,859 | 85.0 kb | Distal (>10kb) Multiome | 409 | |
| chr3:184,236,026–184,236,474 | 78.4 kb | Distal (>10kb) Multiome | 41 | |
| chr3:184,241,140–184,242,189 | 72.8 kb | Distal (>10kb) Multiome | 700 | |
| chr3:184,248,652–184,250,567 | 64.9 kb | Distal (>10kb) Multiome | 1061 | |
| chr3:184,259,723–184,260,497 | 54.5 kb | Distal (>10kb) Multiome | 318 | |
| chr3:184,260,815–184,262,571 | 52.9 kb | Distal (>10kb) Multiome | 511 | |
| chr3:184,298,591–184,299,718 | 15.4 kb | Distal (>10kb) Multiome | 768 | |
| chr3:184,314,169–184,315,514 | 45 bp | At TSS Multiome | 761 | |
| chr3:184,335,265–184,337,276 | 21.4 kb | Distal (>10kb) Multiome | 796 | |
| chr3:184,338,471–184,338,964 | 24.2 kb | Distal (>10kb) Multiome | 203 | |
| chr3:184,361,172–184,363,709 | 48.7 kb | Distal (>10kb) Multiome | 1034 | |
| chr3:184,379,849–184,380,935 | 65.7 kb | Distal (>10kb) Multiome | 242 | |
| chr3:184,513,458–184,514,228 | 199.3 kb | Distal (>10kb) Multiome | 391 | |
| chr3:184,525,317–184,526,423 | 211.5 kb | Distal (>10kb) Multiome | 281 | |
| chr3:184,558,752–184,559,234 | 244.4 kb | Distal (>10kb) Multiome | 58 | |
| chr3:184,561,059–184,562,613 | 247.1 kb | Distal (>10kb) Multiome | 671 | |
| chr3:184,568,694–184,569,481 | 254.6 kb | Distal (>10kb) Multiome | 342 | |
| chr3:184,574,449–184,575,341 | 260.4 kb | Distal (>10kb) Multiome | 164 | |
| chr3:184,583,141–184,585,336 | 270.2 kb | Distal (>10kb) Multiome | 270 | |
| chr3:184,601,537–184,603,254 | 287.7 kb | Distal (>10kb) Multiome | 179 | |
| chr3:184,603,592–184,605,089 | 290.1 kb | Distal (>10kb) Multiome | 321 |
Genomic view of the EIF4G1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.